干旱区生态环境知识资源中心(Arid): Development and validation of a solid-phase extraction method coupled with LC-MS/MS for the simultaneous determination of 16 antibiotic residues in duck meat

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DOI	10.1002/bmc.4501
	Development and validation of a solid-phase extraction method coupled with LC-MS/MS for the simultaneous determination of 16 antibiotic residues in duck meat
	Zheng, Weijia 1; Abd El-Aty, A. M.2,3; Kim, Seong-Kwan 1; Choi, Jeong-Min 1; Park, Da-Hee 1; Yoo, Kyung-Hee 1; Kang, Young-Sun 1,4; Jeon, Jong-Sup 5; Hacimuftuoglu, Ahmet 3; Shim, Jae-Han 6; Shin, Ho-Chul 1
通讯作者	Abd El-Aty, A. M. ; Shin, Ho-Chul
来源期刊	BIOMEDICAL CHROMATOGRAPHY
ISSN	0269-3879
EISSN	1099-0801
出版年	2019
卷号	33 期号:5
英文摘要	The present study was carried out to determine 16 antibiotics belonging to seven different groups (tetracyclines, sulfonamides, penicillins, fluoroquinolones, macrolides, lincosamides and trimethoprims) in duck meat. A solid-phase extraction method based on Oasis HLB cartridges coupled with liquid chromatography-electrospray ionization tandem mass spectrometry was developed. Solutions of 0.1m ethylenediaminetetraacetic acid disodium salt and 2% trifluoroacetic acid were used for the preliminary extraction of the target antibiotics from duck meat and n-hexane was used for purification prior to solid-phase extraction. Mobile phases composed of 0.1% trifluoroacetic acid in distilled water (solvent A) and 0.1% trifluoroacetic acid in methanol (solvent B), combined with a reversed-phase C-18 analytical column, provided the optimal separation and signal intensity. The linearity of the method was assessed using six concentrations (5, 10, 20, 30, 40, and 50g/kg), and the recoveries, which were calculated at three spiking concentrations (5, 10 and 20g/kg), were in the range 69.8-103.3% with relative standard deviations (RSDs) 6.9% for the 16 tested antibiotics. Matrix effects ranging from -47.2 to -13.5% were observed for all the analytes, and the limits of quantitation (LOQ), which ranged from 4.93 to 26.21g/kg, were much lower than the maximum residue limits (MRLs) set by various regulatory authorities. Ten samples from a market were tested, and none of the target analytes were detected. Thus, a simple and versatile protocol has been developed to detect and quantify 16 antibiotics in duck meat samples.
英文关键词	analysis antibiotics duck meat LC-MS MS residues solid-phase extraction
类型	Article
语种	英语
国家	South Korea ; Egypt ; Turkey
收录类别	SCI-E
WOS记录号	WOS:000467964700001
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; MULTICLASS DETERMINATION ; MASS-SPECTROMETRY ; WHITE MEAT ; HONEY ; TETRACYCLINES ; PENICILLINS ; TYLOSIN ; RED
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214656
作者单位	1.Konkuk Univ, Coll Vet Med, Dept Vet Pharmacol & Toxicol, Seoul 143701, South Korea; 2.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt; 3.Ataturk Univ, Med Fac, Dept Med Pharmacol, Erzurum, Turkey; 4.Konkuk Univ, Dept Biomed Sci & Technol, Seoul, South Korea; 5.Gyeonggi Prov Inst Hlth & Environm, Publ Hlth Res Div, Suwon, Gyeonggi Do, South Korea; 6.Chonnam Natl Univ, Coll Agr & Life Sci, Nat Prod Chem Lab, Gwangju, South Korea
推荐引用方式 GB/T 7714	Zheng, Weijia,Abd El-Aty, A. M.,Kim, Seong-Kwan,et al. Development and validation of a solid-phase extraction method coupled with LC-MS/MS for the simultaneous determination of 16 antibiotic residues in duck meat[J],2019,33(5).
APA	Zheng, Weijia.,Abd El-Aty, A. M..,Kim, Seong-Kwan.,Choi, Jeong-Min.,Park, Da-Hee.,...&Shin, Ho-Chul.(2019).Development and validation of a solid-phase extraction method coupled with LC-MS/MS for the simultaneous determination of 16 antibiotic residues in duck meat.BIOMEDICAL CHROMATOGRAPHY,33(5).
MLA	Zheng, Weijia,et al."Development and validation of a solid-phase extraction method coupled with LC-MS/MS for the simultaneous determination of 16 antibiotic residues in duck meat".BIOMEDICAL CHROMATOGRAPHY 33.5(2019).