干旱区生态环境知识资源中心(Arid): Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine

Arid

DOI	10.1155/2019/5921725
	Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine
	Chen, Xinlian 1,2; Cui, Yingxian 1,2; Nie, Liping 1,2; Hu, Haoyu 3; Xu, Zhichao 1,2; Sun, Wei 3; Gao, Ting 4; Song, Jingyuan 1,2; Yao, Hui 1,2
通讯作者	Yao, Hui
来源期刊	BIOMED RESEARCH INTERNATIONAL
ISSN	2314-6133
EISSN	2314-6141
出版年	2019
卷号	2019
英文摘要	Ephedrae Herba and Ephedrae Radix et Rhizoma (Mahuang) have been used as Chinese herbal medicines. Ephedra plants mainly live in deserts and have good governance of desertification. Despite their important medicinal and environmental protection value, dietary supplements containing ephedrine from Ephedra species may threaten the health of people. Morphological resemblance amongst species causes difficulty in identifying the original species of Ephedra herbs. Chloroplast (CP) genome shows good prospects in identification and phylogenetic analysis. This study introduced the structures of the CP genomes of three Ephedra species and analysed their phylogenetic relationships. Three complete CP genomes of Ephedra showed four-part annular structures, namely, two single-copy regions and two inverted repeat regions. The entire CP genomes of three Ephedra species in terms of size were 109,550 bp (E. sinica), 109,667 bp (E. intermedia), and 109,558 bp (E. equisetina). Each CP genome of the three Ephedra species encoded 118 genes, including 73 protein-coding genes, 37 tRNA genes and 8 ribosomal RNA genes. Eleven high-variation regions were screened through mVISTA to be potential specific DNA barcodes for identifying Ephedra species. Maximum likelihood and maximum parsimony trees showed that CP genomes could be used to identify Ephedra species. The Ephedra species had a close phylogenetic relationship with Gnetum species and Welwitschia mirabilis. This research provided valuable information for the identification and phylogenetic analysis of gymnosperms and drug safety of Ephedra.
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Green Published, gold, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000461611100001
WOS关键词	INVERTED REPEAT ; SEQUENCE ; PLANTS ; DNA ; PERFORMANCE ; GNETALES ; GENETICS ; SINICA ; REGION
WOS类目	Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
WOS研究方向	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214654
作者单位	1.Chinese Acad Med Sci, Inst Med Plant Dev, Minist Educ, Engn Res Ctr Tradit Chinese Med Resource, Beijing 100193, Peoples R China; 2.Peking Union Med Coll, Beijing 100193, Peoples R China; 3.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China; 4.Qingdao Agr Univ, Coll Life Sci, Key Lab Plant Biotechnol Univ Shandong Prov, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xinlian,Cui, Yingxian,Nie, Liping,et al. Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine[J],2019,2019.
APA	Chen, Xinlian.,Cui, Yingxian.,Nie, Liping.,Hu, Haoyu.,Xu, Zhichao.,...&Yao, Hui.(2019).Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine.BIOMED RESEARCH INTERNATIONAL,2019.
MLA	Chen, Xinlian,et al."Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine".BIOMED RESEARCH INTERNATIONAL 2019(2019).