干旱区生态环境知识资源中心(Arid): Comparison of different PCR protocols and respective primer sets from pool of TAMS 1 gene for diagnosis of calf theileriosis from semi arid India

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DOI	10.1016/j.biologicals.2018.12.004
	Comparison of different PCR protocols and respective primer sets from pool of TAMS 1 gene for diagnosis of calf theileriosis from semi arid India
	Paliwal, Sanjhi ; Shanker, Daya ; Sudan, Vikrant 1; Kumar, Sanjeev ; Srivastava, Mukesh ; Gupta, Kapil Kumar
通讯作者	Sudan, Vikrant
来源期刊	BIOLOGICALS
ISSN	1045-1056
EISSN	1095-8320
出版年	2019
卷号	57 页码:50-54
英文摘要	The polymorphic nature of Theileria annulata merozoite surface antigen (TAMS 1) attributes to limitation in PCR based detection of various T. annulata genotypes present in different geographical domains across the globe. Multiple reports of failure of detection of tropical theileriosis using classical N516/517 primer set in the studied area were noticed. Hence, three single PCR protocols using N516/517, TAMS FIR and NTA F/R primer sets encoding different portions of TAMS 1 gene and two nested protocols, using combinations of these three primers, were compared to find out the most suitable primer set for diagnosis of calf theileriosis in studied area. The studied area constitutes the semi-arid theileriosis endemic area of Northern India. The various PCR protocols were tested on 75 clinically confirmed cases of calf theileriosis. Alongside, 25 confirmed theileriosis negative blood samples and DNA of other haemoprotozoa were also tested for specificity of these primer sets. Results revealed that the primer set NTA F/R to be more suitable in detecting the circulating T. annulata genotypes in the studied area in comparison to the classical N516/517 primer set. None of the primers gave false positive amplification with negative samples and/or DNA of other haemoprotozoa.
英文关键词	Theileria annulata Theileria annulata merozoite surface gene (TAMS 1) polymorphism PCR
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000457513400007
WOS关键词	PHYLOGENETIC ANALYSIS ; DUPLEX PCR ; ANNULATA ; CATTLE ; ASSAY
WOS类目	Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Pharmacology & Pharmacy
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214645
作者单位	1.UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vig, Mathura 281001, India; 2.Go Anusandhan Sansthan DUVASU, Mathura 281001, India
推荐引用方式 GB/T 7714	Paliwal, Sanjhi,Shanker, Daya,Sudan, Vikrant,et al. Comparison of different PCR protocols and respective primer sets from pool of TAMS 1 gene for diagnosis of calf theileriosis from semi arid India[J],2019,57:50-54.
APA	Paliwal, Sanjhi,Shanker, Daya,Sudan, Vikrant,Kumar, Sanjeev,Srivastava, Mukesh,&Gupta, Kapil Kumar.(2019).Comparison of different PCR protocols and respective primer sets from pool of TAMS 1 gene for diagnosis of calf theileriosis from semi arid India.BIOLOGICALS,57,50-54.
MLA	Paliwal, Sanjhi,et al."Comparison of different PCR protocols and respective primer sets from pool of TAMS 1 gene for diagnosis of calf theileriosis from semi arid India".BIOLOGICALS 57(2019):50-54.