干旱区生态环境知识资源中心(Arid): Suitable reference genes for real-time quantitative PCR in Salsola laricifilia under five abiotic stresses

Arid

DOI	10.32615/bp.2019.044
	Suitable reference genes for real-time quantitative PCR in Salsola laricifilia under five abiotic stresses
	Zhang, Y. -F.1; Wen, Z. -B.1; Wang, Y.1,2; Wang, Y. -L.1,2; Feng, Y.1
通讯作者	Wen, Z. -B.
来源期刊	BIOLOGIA PLANTARUM
ISSN	0006-3134
EISSN	1573-8264
出版年	2019
卷号	63 页码:380-387
英文摘要	Salsola laricifolia, a typical C-3-C-4 intermediate desert plant, is an important for understanding gene evolution and mechanisms for drought resistance. The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a preferred choice for gene expression studies, but it requires stable reference genes for normalization. Therefore, we tested the expression stability of five candidate reference genes in S. laricifolia: EF1 alpha (elongation factor 1-alpha), ACT (actin), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), TUB (tubulin), and 18S (18S ribosomal RNA). The expressions were tested in different tissues and under five stresses caused by abscisic acid (ABA), NaCl, NaHCO3, darkness, and osmotic stress (polyethylene glycol 6000, PEG). Four commonly used software programs (geNorm, NormFinder, BestKeeper, and RefFinder) were used. The results show the following most stable reference genes: GAPDH for ABA and dark treatments; EF1a for NaCl, PEG; and all samples; TUB for NaHCO3; and 18S for the controls. The ACT was not ranked first in any group, and was the least stable reference gene under the dark, NaHCO3, and PEG. Moreover, pairwise analysis by the geNorm algorithm shows that two best reference genes were 18S and EF1a for the controls, GAPDH, and 18S for the ABA and dark treatments, EF1a and TUB for the NaCl treatment, TUB and 18S for the NaHCO3 treatment, EF1a and GAPDH for the PEG treatment, and EF1a and 18S for all samples. The reference genes for RT-qPCR in S. laricifolia identified in our study will facilitate future work on targeted gene expression.
英文关键词	abscisic acid C-3-C-4 intermediate species NaCl NaHCO3 polyethylene glycol RT-qPCR normalization
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000466354600046
WOS关键词	POLYMERASE-CHAIN-REACTION ; SUPERIOR REFERENCE GENES ; TRANSCRIPT NORMALIZATION ; HOUSEKEEPING GENES ; QRT-PCR ; RT-QPCR ; SELECTION ; IDENTIFICATION ; EXPRESSION ; QUANTIFICATION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	中国科学院新疆生态与地理研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214618
作者单位	1.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Y. -F.,Wen, Z. -B.,Wang, Y.,et al. Suitable reference genes for real-time quantitative PCR in Salsola laricifilia under five abiotic stresses[J]. 中国科学院新疆生态与地理研究所,2019,63:380-387.
APA	Zhang, Y. -F.,Wen, Z. -B.,Wang, Y.,Wang, Y. -L.,&Feng, Y..(2019).Suitable reference genes for real-time quantitative PCR in Salsola laricifilia under five abiotic stresses.BIOLOGIA PLANTARUM,63,380-387.
MLA	Zhang, Y. -F.,et al."Suitable reference genes for real-time quantitative PCR in Salsola laricifilia under five abiotic stresses".BIOLOGIA PLANTARUM 63(2019):380-387.