Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.32615/bp.2019.044 |
Suitable reference genes for real-time quantitative PCR in Salsola laricifilia under five abiotic stresses | |
Zhang, Y. -F.1; Wen, Z. -B.1; Wang, Y.1,2; Wang, Y. -L.1,2; Feng, Y.1 | |
通讯作者 | Wen, Z. -B. |
来源期刊 | BIOLOGIA PLANTARUM
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ISSN | 0006-3134 |
EISSN | 1573-8264 |
出版年 | 2019 |
卷号 | 63页码:380-387 |
英文摘要 | Salsola laricifolia, a typical C-3-C-4 intermediate desert plant, is an important for understanding gene evolution and mechanisms for drought resistance. The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a preferred choice for gene expression studies, but it requires stable reference genes for normalization. Therefore, we tested the expression stability of five candidate reference genes in S. laricifolia: EF1 alpha (elongation factor 1-alpha), ACT (actin), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), TUB (tubulin), and 18S (18S ribosomal RNA). The expressions were tested in different tissues and under five stresses caused by abscisic acid (ABA), NaCl, NaHCO3, darkness, and osmotic stress (polyethylene glycol 6000, PEG). Four commonly used software programs (geNorm, NormFinder, BestKeeper, and RefFinder) were used. The results show the following most stable reference genes: GAPDH for ABA and dark treatments; EF1a for NaCl, PEG; and all samples; TUB for NaHCO3; and 18S for the controls. The ACT was not ranked first in any group, and was the least stable reference gene under the dark, NaHCO3, and PEG. Moreover, pairwise analysis by the geNorm algorithm shows that two best reference genes were 18S and EF1a for the controls, GAPDH, and 18S for the ABA and dark treatments, EF1a and TUB for the NaCl treatment, TUB and 18S for the NaHCO3 treatment, EF1a and GAPDH for the PEG treatment, and EF1a and 18S for all samples. The reference genes for RT-qPCR in S. laricifolia identified in our study will facilitate future work on targeted gene expression. |
英文关键词 | abscisic acid C-3-C-4 intermediate species NaCl NaHCO3 polyethylene glycol RT-qPCR normalization |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
开放获取类型 | gold |
收录类别 | SCI-E |
WOS记录号 | WOS:000466354600046 |
WOS关键词 | POLYMERASE-CHAIN-REACTION ; SUPERIOR REFERENCE GENES ; TRANSCRIPT NORMALIZATION ; HOUSEKEEPING GENES ; QRT-PCR ; RT-QPCR ; SELECTION ; IDENTIFICATION ; EXPRESSION ; QUANTIFICATION |
WOS类目 | Plant Sciences |
WOS研究方向 | Plant Sciences |
来源机构 | 中国科学院新疆生态与地理研究所 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/214618 |
作者单位 | 1.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Y. -F.,Wen, Z. -B.,Wang, Y.,et al. Suitable reference genes for real-time quantitative PCR in Salsola laricifilia under five abiotic stresses[J]. 中国科学院新疆生态与地理研究所,2019,63:380-387. |
APA | Zhang, Y. -F.,Wen, Z. -B.,Wang, Y.,Wang, Y. -L.,&Feng, Y..(2019).Suitable reference genes for real-time quantitative PCR in Salsola laricifilia under five abiotic stresses.BIOLOGIA PLANTARUM,63,380-387. |
MLA | Zhang, Y. -F.,et al."Suitable reference genes for real-time quantitative PCR in Salsola laricifilia under five abiotic stresses".BIOLOGIA PLANTARUM 63(2019):380-387. |
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