干旱区生态环境知识资源中心(Arid): Dried Biofilms of Desert Strains of Chroococcidiopsis Survived Prolonged Exposure to Space and Mars-like Conditions in Low Earth Orbit

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DOI	10.1089/ast.2018.1900
	Dried Biofilms of Desert Strains of Chroococcidiopsis Survived Prolonged Exposure to Space and Mars-like Conditions in Low Earth Orbit
	Billi, Daniela 1; Staibano, Clelia 1; Verseux, Cyprien 1; Fagliarone, Claudia 1; Mosca, Claudia 1; Baque, Mickael 2; Rabbow, Elke 3; Rettberg, Petra 3
通讯作者	Billi, Daniela
来源期刊	ASTROBIOLOGY
ISSN	1531-1074
EISSN	1557-8070
出版年	2019
卷号	19 期号:8 页码:1008-1017
英文摘要	Dried biofilms and dried multilayered planktonic counterparts obtained from three desert strains of Chroococcidiopsis were exposed to low Earth conditions by using the EXPOSE-R2 facility outside the International Space Station. During the space mission, samples in Tray 1 (space vacuum and solar radiation, from lambda approximate to 110 nm) and Tray 2 (Mars-like UV flux, lambda > 200 nm and Mars-like atmosphere) received total UV (200-400 nm) fluences of about 4.58 x 10(2) kJ/m(2) and 4.92 x 10(2) kJ/m(2), respectively, and 0.5 Gy of cosmic ionizing radiation. Postflight analyses were performed on 2.5-year-old samples due to the space mission duration, from launch to sample return to the lab. The occurrence of survivors was determined by evaluating cell division upon rehydration and damage to the genome and photosynthetic apparatus by polymerase chain reaction-stop assays and confocal laser scanning microscopy. Biofilms recovered better than their planktonic counterparts, accumulating less damage not only when exposed to UV radiation under space and Mars-like conditions but also when exposed in dark conditions to low Earth conditions and laboratory control conditions. This suggests that, despite the shielding provided by top-cell layers being sufficient for a certain degree of survival of the multilayered planktonic samples, the enhanced survival of biofilms was due to the presence of abundant extracellular polymeric substances and to additional features acquired upon drying.
英文关键词	Cyanobacteria Mars-like Space Biofilms Planktonic EXPOSE
类型	Article
语种	英语
国家	Italy ; Germany
收录类别	SCI-E
WOS记录号	WOS:000478581200005
WOS关键词	CYANOBACTERIUM CHROOCOCCIDIOPSIS ; RADIATION-RESISTANCE ; EXPOSE-R2 MISSION ; SIMULATED SPACE ; DESICCATION ; LIFE ; ENVIRONMENT ; TOOL ; HOT
WOS类目	Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
WOS研究方向	Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214422
作者单位	1.Univ Roma Tor Vergata, Dept Biol, Via Ric Sci, I-00133 Rome, Italy; 2.German Aerosp Ctr DLR, Inst Planetary Res Management & Infrastruct, Astrobiol Labs, Res Grp, Berlin, Germany; 3.German Aerosp Ctr DLR, Inst Aerosp Med, Cologne, Germany
推荐引用方式 GB/T 7714	Billi, Daniela,Staibano, Clelia,Verseux, Cyprien,et al. Dried Biofilms of Desert Strains of Chroococcidiopsis Survived Prolonged Exposure to Space and Mars-like Conditions in Low Earth Orbit[J],2019,19(8):1008-1017.
APA	Billi, Daniela.,Staibano, Clelia.,Verseux, Cyprien.,Fagliarone, Claudia.,Mosca, Claudia.,...&Rettberg, Petra.(2019).Dried Biofilms of Desert Strains of Chroococcidiopsis Survived Prolonged Exposure to Space and Mars-like Conditions in Low Earth Orbit.ASTROBIOLOGY,19(8),1008-1017.
MLA	Billi, Daniela,et al."Dried Biofilms of Desert Strains of Chroococcidiopsis Survived Prolonged Exposure to Space and Mars-like Conditions in Low Earth Orbit".ASTROBIOLOGY 19.8(2019):1008-1017.