干旱区生态环境知识资源中心(Arid): On-Site Analysis of Bacterial Communities of the Ultraoligotrophic South Pacific Gyre

Arid

DOI	10.1128/AEM.00184-19
	On-Site Analysis of Bacterial Communities of the Ultraoligotrophic South Pacific Gyre
	Reintjes, Greta 1; Tegetmeyer, Halina E.1,2,3; Buergisser, Miriam 1; Orlic, Sandi 4,5; Tews, Ivo 6; Zubkov, Mikhail 7; Voss, Daniela 8; Zielinski, Oliver 8; Quast, Christian 1; Gloeckner, Frank Oliver 1,9; Amann, Rudolf 1; Ferdelman, Timothy G.1; Fuchs, Bernhard M.1
通讯作者	Reintjes, Greta
来源期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
EISSN	1098-5336
出版年	2019
卷号	85 期号:14
英文摘要	The South Pacific Gyre (SPG) covers 10% of the ocean's surface and is often regarded as a marine biological desert. To gain an on-site overview of the remote, ultraoligotrophic microbial community of the SPG, we developed a novel on-board analysis pipeline, which combines next-generation sequencing with fluorescence in situ hybridization and automated cell enumeration. We tested the pipeline during the SO-245 UltraPac cruise from Chile to New Zealand and found that the overall microbial community of the SPG was highly similar to those of other oceanic gyres. The SPG was dominated by 20 major bacterial clades, including SAR11, SAR116, the AEGEAN-169 marine group, SAR86, Prochlorococcus, SAR324, SAR406, and SAR202. Most of the bacterial clades showed a strong vertical (20m to 5,000 m), but only a weak longitudinal (80 degrees W to 160 degrees W), distribution pattern. Surprisingly, in the central gyre, Prochlorococcus, the dominant photosynthetic organism, had only low cellular abundances in the upper waters (20 to 80 m) and was more frequent around the 1% irradiance zone (100 to 150 m). Instead, the surface waters of the central gyre were dominated by the SAR11, SAR86, and SAR116 clades known to harbor light-driven proton pumps. The alphaproteobacterial AEGEAN-169 marine group was particularly abundant in the surface waters of the central gyre, indicating a potentially interesting adaptation to ultraoligotrophic waters and high solar irradiance. In the future, the newly developed community analysis pipeline will allow for on-site insights into a microbial community within 35 h of sampling, which will permit more targeted sampling efforts and hypothesis-driven research. IMPORTANCE The South Pacific Gyre, due to its vast size and remoteness, is one of the least-studied oceanic regions on earth. However, both remote sensing and in situ measurements indicated that the activity of its microbial community contributes significantly to global biogeochemical cycles. Presented here is an unparalleled investigation of the microbial community of the SPG from 20- to 5,000-m depths covering a geographic distance of similar to 7,000 km. This insight was achieved through the development of a novel on-board analysis pipeline, which combines next-generation sequencing with fluorescence in situ hybridization and automated cell enumeration. The pipeline is well comparable to onshore systems based on the Illumina platforms and yields microbial community data in less than 35 h after sampling. Going forward, the ability to gain on-site knowledge of a remote microbial community will permit hypothesis-driven research, through the generation of novel scientific questions and subsequent additional targeted sampling efforts.
英文关键词	AEGEAN-169 biogeography microbial community structure Prochlorococcus South Pacific Subtropical Gyre
类型	Article
语种	英语
国家	Germany ; Croatia ; England
开放获取类型	Green Published, Bronze, Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000476468600001
WOS关键词	NORTH-ATLANTIC ; PICOPLANKTON POPULATIONS ; IN-SITU ; OCEAN ; PROCHLOROCOCCUS ; SAR11 ; GENOMES ; DISTRIBUTIONS ; DIVERSITY ; WATERS
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214221
作者单位	1.Max Planck Inst Marine Microbiol, Bremen, Germany; 2.Alfred Wegener Inst, Helmholtz Ctr Polar & Marine Res, Bremerhaven, Germany; 3.Bielefeld Univ, Ctr Biotechnol, Bielefeld, Germany; 4.Inst Ruder Boskovic, Zagreb, Croatia; 5.Ctr Excellence Sci & Technol Integrat Mediterran, Zagreb, Croatia; 6.Univ Southampton, Inst Life Sci, Biol Sci, Southampton, Hants, England; 7.Natl Oceanog Ctr, Southampton, Hants, England; 8.Carl von Ossietzky Univ Oldenburg, Inst Chem & Biol Marine Environm, Oldenburg, Germany; 9.Jacobs Univ, Bremen, Germany
推荐引用方式 GB/T 7714	Reintjes, Greta,Tegetmeyer, Halina E.,Buergisser, Miriam,et al. On-Site Analysis of Bacterial Communities of the Ultraoligotrophic South Pacific Gyre[J],2019,85(14).
APA	Reintjes, Greta.,Tegetmeyer, Halina E..,Buergisser, Miriam.,Orlic, Sandi.,Tews, Ivo.,...&Fuchs, Bernhard M..(2019).On-Site Analysis of Bacterial Communities of the Ultraoligotrophic South Pacific Gyre.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,85(14).
MLA	Reintjes, Greta,et al."On-Site Analysis of Bacterial Communities of the Ultraoligotrophic South Pacific Gyre".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 85.14(2019).