干旱区生态环境知识资源中心(Arid): Analysis of the bacterial communities and endosymbionts of natural populations of Bemisia tabaci in several crop fields from Mexico semi-arid zone

Arid

DOI	10.1007/s13213-019-01483-6
	Analysis of the bacterial communities and endosymbionts of natural populations of Bemisia tabaci in several crop fields from Mexico semi-arid zone
	Maria Goretty, Caamal-Chan 1; Abraham, Loera-Muro 1; Thelma, Castellanos 2; Carlos Julian, Aguilar-Martinez 3; Miguel David, Marfil-Santana 4; Aaron, Barraza 1
通讯作者	Aaron, Barraza
来源期刊	ANNALS OF MICROBIOLOGY
ISSN	1590-4261
EISSN	1869-2044
出版年	2019
卷号	69 期号:9 页码:909-922
英文摘要	Purpose Bemisia tabaci (Aleyrodidae family) is an insect vector of plant viruses that affects a wide variety of crops around the world. In the following study, we analyzed the variation in the bacterial communities associated with natural populations of B. tabaci (MEAM1) of four different crops from six regions from Mexico semi-arid zone (Baja California Sur). Methods PCR was used to amplify the mitochondrial cytochrome oxidase I gene (mtCOI), and then to carry out the phylogenetic analysis for genetic identification of the isolated B. tabaci. Next generation sequencing coupled with 16S metagenomic analysis was applied in order to characterize B. tabaci inner microbial community. Finally, bacterial obligate symbiont and facultative symbiont were confirmed by PCR amplification and by phylogenetic analysis. Results Ours results pointed toward that B. tabaci MEAM1 inner bacterial communities were predominantly structured by Proteobacteria phylum. Moreover, the most represented endosymbionts were the obligate endosymbiont from the genus Candidatus Portiera, as well as two facultative symbionts belonging to genera Rickettsia and Hamiltonella; both obligate and facultative endosymbionts were present for all samples, and their relative abundance varied was crop-independent. Conclusions Geographic localization and insect diet play a central role to maintain bacterial community structure of the B. tabaci MEAM1 whitefly at phylum taxonomic level. Agricultural practices were a factor that affected samples of bacterial community structure similarities, reflected in samples clustering. Host plants that are part of B. tabaci diet did not influence directly, in spite of sap nutrient differences, into obligate and facultative endosymbionts relative abundance.
英文关键词	Bemisia tabaci Bacterial communities Endosymbiont Environmental factors
类型	Article
语种	英语
国家	Mexico
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000480738100004
WOS关键词	RESISTANCE ; SIZE
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
来源机构	Universidad Nacional Autónoma de México
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214189
作者单位	1.Inst Politecn Nacl, CONACYT Ctr Invest Biolog Noroeste SC, 195 Playa Palo Santa Rita Sur, La Paz 23096, Bcs, Mexico; 2.Inst Politecn Nacl, Ctr Invest Biol Noroeste, 195 Playa Palo Santa Rita Sur, La Paz 23096, Bcs, Mexico; 3.Inst Tecnol La Paz, Blvd Forjadores Baja California Sur 4720, La Paz 23080, Bcs, Mexico; 4.Univ Nacl Autonoma Mexico, Fac Quim, Unidad Sisal, Sisal 97356, Yucatan, Mexico
推荐引用方式 GB/T 7714	Maria Goretty, Caamal-Chan,Abraham, Loera-Muro,Thelma, Castellanos,et al. Analysis of the bacterial communities and endosymbionts of natural populations of Bemisia tabaci in several crop fields from Mexico semi-arid zone[J]. Universidad Nacional Autónoma de México,2019,69(9):909-922.
APA	Maria Goretty, Caamal-Chan,Abraham, Loera-Muro,Thelma, Castellanos,Carlos Julian, Aguilar-Martinez,Miguel David, Marfil-Santana,&Aaron, Barraza.(2019).Analysis of the bacterial communities and endosymbionts of natural populations of Bemisia tabaci in several crop fields from Mexico semi-arid zone.ANNALS OF MICROBIOLOGY,69(9),909-922.
MLA	Maria Goretty, Caamal-Chan,et al."Analysis of the bacterial communities and endosymbionts of natural populations of Bemisia tabaci in several crop fields from Mexico semi-arid zone".ANNALS OF MICROBIOLOGY 69.9(2019):909-922.