干旱区生态环境知识资源中心(Arid): Ultrahigh-throughput absolute quantitative analysis of linezolid in human plasma by direct analysis in real time mass spectrometry without chromatographic separation and its application to a pharmacokinetic study

Arid

DOI	10.1007/s00216-019-01891-2
	Ultrahigh-throughput absolute quantitative analysis of linezolid in human plasma by direct analysis in real time mass spectrometry without chromatographic separation and its application to a pharmacokinetic study
	Yin, Lei 1,2,3; Feng, Yixuan 2; Tong, Jin 2; Guo, Zhiqiong 2; Zhang, Yuyao 2; Zhang, Qi 2; Sun, Yantong 4; Fawcett, J. Paul 5; Gu, Jingkai 1,2,6
通讯作者	Gu, Jingkai
来源期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN	1618-2642
EISSN	1618-2650
出版年	2019
卷号	411 期号:20 页码:5139-5148
英文摘要	Therapeutic drug monitoring (TDM) is necessary in the clinical management of linezolid to improve its efficacy and reduce the risk of time- and dose-dependent toxicity. A novel and ultrahigh-throughput analytical method for the determination of linezolid in human plasma was developed based on direct analysis in real-time tandem mass spectrometry (DART-MS/MS) without chromatographic separation. After solid-phase extraction with Waters Oasis HLB, the linezolid and internal standard linezolid-d(3) were detected by positive ion electrospray ionization followed by multiple reaction monitoring (MRM) of the transition at m/z 338.1 -> 296.2 and 341.2 -> 297.3, respectively. The use of DART-MS obviates the need for chromatographic separation and allowed determination of linezolid in a total run time of only 24s per sample. The method was linear in the concentration range 0.20-25 mu gmL(-1) with intraday and interday precision <14.5% and accuracy ranging from -3.85% to 12.7%. The method was successfully applied to a pharmacokinetic study of linezolid in healthy male volunteers after oral administration of a 600mg tablet. DART-MS/MS provides a rapid and sensitive method for the determination of linezolid that does not require chromatographic separation. It is eminently suitable to meet the high-throughput challenge of clinical TDM.
英文关键词	LC-MS MS DART Linezolid High throughput Pharmacokinetics
类型	Article
语种	英语
国家	Peoples R China ; USA ; New Zealand
收录类别	SCI-E
WOS记录号	WOS:000475515600013
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; DART-MS ; IN-VITRO ; MS/MS METHOD ; HPLC ASSAY ; SAFETY ; CIPROFLOXACIN ; TUBERCULOSIS ; VALIDATION ; QUANTIFICATION
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
来源机构	University of Arizona
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214145
作者单位	1.Jilin Univ, Hosp 1, Res Inst Translat Med, Dongminzhu St, Changchun 130061, Jilin, Peoples R China; 2.Jilin Univ, Sch Life Sci, Res Ctr Drug Metab, Qianjin St, Changchun 130012, Jilin, Peoples R China; 3.Univ Arizona, Coll Pharm, Tucson, AZ 85721 USA; 4.Jilin Univ, Sch Pharmaceut Sci, Changchun 130012, Jilin, Peoples R China; 5.Univ Otago, Sch Pharm, POB 56, Dunedin 9054, New Zealand; 6.Beijing Inst Modern Drug Metab, Beijing 102209, Peoples R China
推荐引用方式 GB/T 7714	Yin, Lei,Feng, Yixuan,Tong, Jin,et al. Ultrahigh-throughput absolute quantitative analysis of linezolid in human plasma by direct analysis in real time mass spectrometry without chromatographic separation and its application to a pharmacokinetic study[J]. University of Arizona,2019,411(20):5139-5148.
APA	Yin, Lei.,Feng, Yixuan.,Tong, Jin.,Guo, Zhiqiong.,Zhang, Yuyao.,...&Gu, Jingkai.(2019).Ultrahigh-throughput absolute quantitative analysis of linezolid in human plasma by direct analysis in real time mass spectrometry without chromatographic separation and its application to a pharmacokinetic study.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(20),5139-5148.
MLA	Yin, Lei,et al."Ultrahigh-throughput absolute quantitative analysis of linezolid in human plasma by direct analysis in real time mass spectrometry without chromatographic separation and its application to a pharmacokinetic study".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.20(2019):5139-5148.