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DOI	10.1007/s00216-018-1451-6
	Silver and gold nanoparticles as multi-chromatic lateral flow assay probes for the detection of food allergens
	Anfossi, Laura 1; Di Nardo, Fabio 1; Russo, Alida 2; Cavalera, Simone 1; Giovannoli, Cristina 1; Spano, Giulia 1; Baumgartner, Sabine 3; Lauter, Kathrin 3; Baggiani, Claudio 1
通讯作者	Anfossi, Laura
来源期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN	1618-2642
EISSN	1618-2650
出版年	2019
卷号	411 期号:9 页码:1905-1913
英文摘要	In this study, we report the simultaneous use of gold and silver nanoparticles to set a multicolor multiplex lateral flow immunoassay (xLFIA). Silver nanoparticles (AgNPs), spherical in shape and characterized by a brilliant yellow color, were obtained by a new viable one-step synthetic protocol. AgNPs were stable over time and acceptably robust to conditions used for fabricating LFIA devices. These AgNPs were employed as a colorimetric probe in combination with two different kinds of gold nanoparticles (AuNPs) to set a visual xLFIA for detecting allergens. Surface plasmon resonance peaks of probes (AgNPs, spherical and desert rose-like AuNPs) were centered at 420, 525, and 620nm, respectively. Therefore, the xLFIA output was easily interpreted through a yellow magenta cyan (YMC) color code. The prospect of the YMC xLFIA was demonstrated by simultaneously detecting three major allergens in bakery products. Antibodies directed towards casein, ovalbumin, and hazelnut allergenic proteins were individually adsorbed onto metal nanoparticles to produce three differently colored specific probes. These were inserted in a LFIA comprising three lines, each responsive for one allergen. The trichromatic xLFIA was able to detect allergenic proteins at levels as low as 0.1mg/l and enabled the easy identification of the allergens in commercial biscuits based on the color of the probes.
英文关键词	Immunochromatographic strip test Multiplex detection Ovalbumin Casein Hazelnut Colorimetry
类型	Article
语种	英语
国家	Italy ; Austria
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000463858200021
WOS关键词	LINKED-IMMUNOSORBENT-ASSAY ; RAPID DETECTION ; COLLOIDAL GOLD ; IMMUNOASSAY STRIP ; TUMOR-MARKERS ; QUANTUM DOTS ; ANTIBIOTICS ; AGENTS
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214144
作者单位	1.Univ Turin, Dept Chem, Via Giuria 5, I-10125 Turin, Italy; 2.Univ Turin, Dept Vet Sci, Turin, Italy; 3.Univ Nat Resources & Life Sci BOKU, Dept Agrobiotechnol Tulln, Ctr Analyt Chem, Konrad Lorenz Str 20, A-3430 Tulln, Austria
推荐引用方式 GB/T 7714	Anfossi, Laura,Di Nardo, Fabio,Russo, Alida,et al. Silver and gold nanoparticles as multi-chromatic lateral flow assay probes for the detection of food allergens[J],2019,411(9):1905-1913.
APA	Anfossi, Laura.,Di Nardo, Fabio.,Russo, Alida.,Cavalera, Simone.,Giovannoli, Cristina.,...&Baggiani, Claudio.(2019).Silver and gold nanoparticles as multi-chromatic lateral flow assay probes for the detection of food allergens.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(9),1905-1913.
MLA	Anfossi, Laura,et al."Silver and gold nanoparticles as multi-chromatic lateral flow assay probes for the detection of food allergens".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.9(2019):1905-1913.