干旱区生态环境知识资源中心(Arid): Bio-inspired photonic crystals for naked eye quantification of nucleic acids

Arid

DOI	10.1039/c9an01042d
	Bio-inspired photonic crystals for naked eye quantification of nucleic acids
	Zhu, Ke 1; Chi, Junjie 1; Zhang, Dagan 1; Ma, Biao 1; Dong, Xing 1; Yang, Jun 2; Zhao, Chao 1; Liu, Hong 1
通讯作者	Zhao, Chao ; Liu, Hong
来源期刊	ANALYST
ISSN	0003-2654
EISSN	1364-5528
出版年	2019
卷号	144 期号:18 页码:5413-5419
英文摘要	Herein, a chip imitating the desert beetle shell was presented for naked eye nucleic acid quantification. The hydrophobic photonic crystal substrate treated by ultraviolet local irradiation could effectively disperse the sample into hundreds of droplets for digital loop-mediated isothermal amplification (dLAMP). Pyrophosphate (PPI), a by-product of the LAMP reaction, combined with magnesium ions to form a poorly soluble precipitate. It could be fixed on a silica substrate due to complexation, resulting in the disappearance of the structural color of the photonic crystals. The number of points without structural color contains the information of the copy number of nucleic acids in the sample. This chip could achieve the naked eye quantitative detection of Salmonella DNA without fluorescence or other chromogenic reagents. Thus, the chip designed in this study can help the development of digital nucleic acid detection under limited resource settings (LRS) and can be suitable for POCT (point of care test) standards.
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000484974900035
WOS关键词	MEDIATED ISOTHERMAL AMPLIFICATION ; DIGITAL PCR ; DNA ; PLATFORM ; CHIP ; RNA
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214140
作者单位	1.Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China; 2.Nanjing Inst Food & Drug Control, Nanjing 210038, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Ke,Chi, Junjie,Zhang, Dagan,et al. Bio-inspired photonic crystals for naked eye quantification of nucleic acids[J],2019,144(18):5413-5419.
APA	Zhu, Ke.,Chi, Junjie.,Zhang, Dagan.,Ma, Biao.,Dong, Xing.,...&Liu, Hong.(2019).Bio-inspired photonic crystals for naked eye quantification of nucleic acids.ANALYST,144(18),5413-5419.
MLA	Zhu, Ke,et al."Bio-inspired photonic crystals for naked eye quantification of nucleic acids".ANALYST 144.18(2019):5413-5419.