干旱区生态环境知识资源中心(Arid): Assessment of pearl millet genotypes for drought stress tolerance at early and late seedling stages

Arid

DOI	10.1007/s11738-019-2831-z
	Assessment of pearl millet genotypes for drought stress tolerance at early and late seedling stages
	Shivhare, Radha 1,2; Lata, Charu 1,2
通讯作者	Lata, Charu
来源期刊	ACTA PHYSIOLOGIAE PLANTARUM
ISSN	0137-5881
EISSN	1861-1664
出版年	2019
卷号	41 期号:3
英文摘要	Pearl millet is one of the most important C-4 panicoid millet crops grown in dry and arid regions of India and West Africa. Drought is one of the major limitations to its growth potential. Therefore, selection of drought-tolerant cultivars through in vitro screening can be an important approach for crop improvement programmes. An experiment was conducted on six pearl millet genotypes with two different levels of osmotic stress treatments, i.e., control and 20% polyethylene glycol (PEG) 6000at early seedling stage for eight seedling traits and proline content, while 30% PEG at late seedling stage for proline and malonaldehyde (MDA) content and gene expression analysis. Analysis of variance (ANOVA) showed significant treatment effects on all early seedling traits with respect to treatment x varietal interaction. Our results showed a relative decline for different seedling traits in individual genotypes with increased osmotic stress. TT-1 showed a maximum reduction in almost all traits while the minimum decline was noted in PRLT2/89-33. Further, proline and MDA accumulation in control and 30% PEG-6000-treated seedlings at late growth stages were in confirmation with the observations recorded at early seedling growth stages. Expression analysis using quantitative real-time (qRT)-PCR of three antioxidative genes namely, ascorbate peroxidase (APX), glutathione reductase (GlutR) and superoxide dismutase (SOD) also confirmed higher osmotic stress tolerance of PRLT2/89-33 seedlings. Overall results suggested that selection for any of the above traits at early or late-seedling growth stages may prove useful for crop improvement programs directed towards developing stress-tolerant cultivars.
英文关键词	Drought Genotypes Germination Osmotic stress Pearl millet Polyethylene glycol
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000460009800001
WOS关键词	GENE-EXPRESSION ; WATER-STRESS ; LIPID-PEROXIDATION ; L. ; GERMINATION ; RESPONSES ; INDEXES ; SORGHUM ; PEG ; PROLINE
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/213862
作者单位	1.CSIR Natl Bot Res Inst, Rana Pratap Marg, Lucknow 226001, Uttar Pradesh, India; 2.Acad Sci & Innovat Res AcSIR, CSIR HRDC Campus,Sect 19, Kamla Nehru Nagar 201002, Ghaziabad, India
推荐引用方式 GB/T 7714	Shivhare, Radha,Lata, Charu. Assessment of pearl millet genotypes for drought stress tolerance at early and late seedling stages[J],2019,41(3).
APA	Shivhare, Radha,&Lata, Charu.(2019).Assessment of pearl millet genotypes for drought stress tolerance at early and late seedling stages.ACTA PHYSIOLOGIAE PLANTARUM,41(3).
MLA	Shivhare, Radha,et al."Assessment of pearl millet genotypes for drought stress tolerance at early and late seedling stages".ACTA PHYSIOLOGIAE PLANTARUM 41.3(2019).