Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1093/treephys/tpx137 |
Transcriptome analysis of Pinus halepensis under drought stress and during recovery | |
Fox, Hagar1,2; Doron-Faigenboim, Adi1; Kelly, Gilor1; Bourstein, Ronny2; Attia, Ziv2; Zhou, Jing1; Moshe, Yosef1; Moshelion, Menachem2; David-Schwartz, Rakefet1 | |
通讯作者 | David-Schwartz, Rakefet |
来源期刊 | TREE PHYSIOLOGY
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ISSN | 0829-318X |
EISSN | 1758-4469 |
出版年 | 2018 |
卷号 | 38期号:3页码:423-441 |
英文摘要 | Forest trees use various strategies to cope with drought stress and these strategies involve complex molecular mechanisms. Pinus halepensis Miller (Aleppo pine) is found throughout the Mediterranean basin and is one of the most drought-tolerant pine species. In order to decipher the molecular mechanisms that P. halepensis uses to withstand drought, we performed large-scale physiological and transcriptome analyses. We selected a mature tree from a semi-arid area with suboptimal growth conditions for clonal propagation through cuttings. We then used a high-throughput experimental system to continuously monitor whole-plant transpiration rates, stomatal conductance and the vapor pressure deficit. The transcriptomes of plants were examined at six physiological stages: pre-stomatal response, partial stomatal closure, minimum transpiration, post-irrigation, partial recovery and full recovery. At each stage, data from plants exposed to the drought treatment were compared with data collected from well-irrigated control plants. A drought-stressed P. halepensis transcriptome was created using paired-end RNA-seq. In total, similar to 6000 differentially expressed, non-redundant transcripts were identified between drought-treated and control trees. Cluster analysis has revealed stress-induced down-regulation of transcripts related to photosynthesis, reactive oxygen species (ROS)-scavenging through the ascorbic acid (AsA)-glutathione cycle, fatty acid and cell wall biosynthesis, stomatal activity, and the biosynthesis of flavonoids and terpenoids. Up-regulated processes included chlorophyll degradation, ROS-scavenging through AsA-independent thiol-mediated pathways, abscisic acid response and accumulation of heat shock proteins, thaumatin and exordium. Recovery from drought induced strong transcription of retrotransposons, especially the retrovirus-related transposon Tnt1-94. The drought-related transcriptome illustrates this species’ dynamic response to drought and recovery and unravels novel mechanisms. |
英文关键词 | conifers drought resistance next generation sequencing retrotransposable elements |
类型 | Article |
语种 | 英语 |
国家 | Israel |
收录类别 | SCI-E |
WOS记录号 | WOS:000427153400009 |
WOS关键词 | THAUMATIN-LIKE PROTEIN ; TOBACCO TNT1 RETROTRANSPOSON ; ABSCISIC-ACID ; ABIOTIC STRESS ; PHYSIOLOGICAL-RESPONSES ; SECONDARY METABOLITES ; GENE-EXPRESSION ; LOBLOLLY-PINE ; ALEPPO PINE ; PHOTOSYNTHETIC ACCLIMATION |
WOS类目 | Forestry |
WOS研究方向 | Forestry |
来源机构 | Hebrew University of Jerusalem |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/213496 |
作者单位 | 1.ARO, Volcani Ctr, Inst Plant Sci, IL-50250 Bet Dagan, Israel; 2.Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Inst Plant Sci & Genet Agr, IL-76100 Rehovot, Israel |
推荐引用方式 GB/T 7714 | Fox, Hagar,Doron-Faigenboim, Adi,Kelly, Gilor,et al. Transcriptome analysis of Pinus halepensis under drought stress and during recovery[J]. Hebrew University of Jerusalem,2018,38(3):423-441. |
APA | Fox, Hagar.,Doron-Faigenboim, Adi.,Kelly, Gilor.,Bourstein, Ronny.,Attia, Ziv.,...&David-Schwartz, Rakefet.(2018).Transcriptome analysis of Pinus halepensis under drought stress and during recovery.TREE PHYSIOLOGY,38(3),423-441. |
MLA | Fox, Hagar,et al."Transcriptome analysis of Pinus halepensis under drought stress and during recovery".TREE PHYSIOLOGY 38.3(2018):423-441. |
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