Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.3390/toxins10040135 |
Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families | |
Strickland, Jason L.1; Mason, Andrew J.2; Rokyta, Darin R.3; Parkinson, Christopher L.1,2,4 | |
通讯作者 | Parkinson, Christopher L. |
来源期刊 | TOXINS |
EISSN | 2072-6651 |
出版年 | 2018 |
卷号 | 10期号:4 |
英文摘要 | Phenotypic diversity generated through altered gene expression is a primary mechanism facilitating evolutionary response in natural systems. By linking the phenotype to genotype through transcriptomics, it is possible to determine what changes are occurring at the molecular level. High phenotypic diversity has been documented in rattlesnake venom, which is under strong selection due to its role in prey acquisition and defense. Rattlesnake venom can be characterized by the presence (Type A) or absence (Type B) of a type of neurotoxic phospholipase A(2) (PLA(2)), such as Mojave toxin, that increases venom toxicity. Mojave rattlesnakes (Crotalus scutulatus), represent this diversity as both venom types are found within this species and within a single panmictic population in the Sonoran Desert. We used comparative venom gland transcriptomics of nine specimens of C. scutulatus from this region to test whether expression differences explain diversity within and between venom types. Type A individuals expressed significantly fewer toxins than Type B individuals owing to the diversity of C-type lectins (CTLs) and snake venom metalloproteinases (SVMPs) found in Type B animals. As expected, both subunits of Mojave toxin were exclusively found in Type A individuals but we found high diversity in four additional PLA(2)s that was not associated with a venom type. Myotoxin a expression and toxin number variation was not associated with venom type, and myotoxin a had the highest range of expression of any toxin class. Our study represents the most comprehensive transcriptomic profile of the venom type dichotomy in rattlesnakes and C. scutulatus. Even intra-specifically, Mojave rattlesnakes showcase the diversity of snake venoms and illustrate that variation within venom types blurs the distinction of the venom dichotomy. |
英文关键词 | C-type lectins hemorrhagic Mojave toxin myotoxin a neurotoxic phospholipase A(2) RNA-seq snake venom metalloproteinases |
类型 | Article |
语种 | 英语 |
国家 | USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000435183700006 |
WOS关键词 | GLAND TRANSCRIPTOMICS ; AMERICAN RATTLESNAKE ; SIGNAL PEPTIDES ; EVOLUTION ; ALIGNMENT ; REVEAL ; POPULATIONS ; PROTEOMICS ; DIVERSITY ; SEQUENCES |
WOS类目 | Food Science & Technology ; Toxicology |
WOS研究方向 | Food Science & Technology ; Toxicology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/213479 |
作者单位 | 1.Univ Cent Florida, Dept Biol, 4110 Libra Dr, Orlando, FL 32816 USA; 2.Clemson Univ, Dept Biol Sci, 190 Collings St, Clemson, SC 29634 USA; 3.Florida State Univ, Dept Biol Sci, 319 Stadium Dr, Tallahassee, FL 32306 USA; 4.Clemson Univ, Dept Forestry & Environm Conservat, 190 Collings St, Clemson, SC 29634 USA |
推荐引用方式 GB/T 7714 | Strickland, Jason L.,Mason, Andrew J.,Rokyta, Darin R.,et al. Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families[J],2018,10(4). |
APA | Strickland, Jason L.,Mason, Andrew J.,Rokyta, Darin R.,&Parkinson, Christopher L..(2018).Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families.TOXINS,10(4). |
MLA | Strickland, Jason L.,et al."Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families".TOXINS 10.4(2018). |
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