Arid
DOI10.3390/toxins10010035
Biological and Proteolytic Variation in the Venom of Crotalus scutulatus scutulatus from Mexico
Borja, Miguel1,2; Neri-Castro, Edgar3; Castaneda-Gaytan, Gamaliel1; Strickland, Jason L.4; Parkinson, Christopher L.5,6; Castaneda-Gaytan, Juan1; Ponce-Lopez, Roberto3; Lomonte, Bruno7; Olvera-Rodriguez, Alejandro3; Alagon, Alejandro3; Perez-Morales, Rebeca2
通讯作者Perez-Morales, Rebeca
来源期刊TOXINS
ISSN2072-6651
出版年2018
卷号10期号:1
英文摘要

Rattlesnake venoms may be classified according to the presence/absence and relative abundance of the neurotoxic phospholipases A(2)s (PLA(2)s), such as Mojave toxin, and snake venom metalloproteinases (SVMPs). In Mexico, studies to determine venom variation in Mojave Rattlesnakes (Crotalus scutulatus scutulatus) are limited and little is known about the biological and proteolytic activities in this species. Tissue (34) and venom (29) samples were obtained from C. s. scutulatus from different locations within their distribution in Mexico. Mojave toxin detection was carried out at the genomic (by PCR) and protein (by ELISA) levels for all tissue and venom samples. Biological activity was tested on representative venoms by measuring LD50 and hemorrhagic activity. To determine the approximate amount of SVMPs, 15 venoms were separated by RP-HPLC and variation in protein profile and proteolytic activity was evaluated by SDS-PAGE (n = 28) and Hide Powder Azure proteolytic analysis (n = 27). Three types of venom were identified in Mexico which is comparable to the intraspecific venom diversity observed in the Sonoran Desert of Arizona, USA: Venom Type A (similar to Type II), with Mojave toxin, highly toxic, lacking hemorrhagic activity, and with scarce proteolytic activity; Type B (similar to Type I), without Mojave toxin, less toxic than Type A, highly hemorrhagic and proteolytic; and Type A + B, containing Mojave toxin, as toxic as venom Type A, variable in hemorrhagic activity and with intermediate proteolytic activity. We also detected a positive correlation between SVMP abundance and hemorrhagic and proteolytic activities. Although more sampling is necessary, our results suggest that venoms containing Mojave toxin and venom lacking this toxin are distributed in the northwest and southeast portions of the distribution in Mexico, respectively, while an intergradation in the middle of both zones is present.


英文关键词Mojave Rattlesnakes Mojave toxin PLA(2)s SVMPs venom phenotypes hemorrhagic activity
类型Article
语种英语
国家Mexico ; USA ; Costa Rica
收录类别SCI-E
WOS记录号WOS:000424096500035
WOS关键词MOJAVE TOXIN ; SNAKE VENOMICS ; DIAMONDBACK RATTLESNAKE ; ACIDIC SUBUNIT ; SEQUENCE ; POPULATIONS ; DISINTEGRIN ; LACKING
WOS类目Food Science & Technology ; Toxicology
WOS研究方向Food Science & Technology ; Toxicology
来源机构Universidad Nacional Autónoma de México
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/213477
作者单位1.Univ Juarez Estado Durango, Fac Ciencias Biol, Ave Univ S-N Fracc Filadelfia, Gomez Palacio 35010, Dgo, Mexico;
2.Univ Juarez Estado Durango, Fac Ciencias Quim, Ave Articulo 123 S-N Fracc Filadelfia, Gomez Palacio 35010, Dgo, Mexico;
3.Univ Nacl Autonoma Mexico, Inst Biotecnol, Ave Univ 2001, Cuernavaca 62210, Mor, Mexico;
4.Univ Cent Florida, Dept Biol, 4000 Cent Florida Blvd, Orlando, FL 32816 USA;
5.Clemson Univ, Dept Biol Sci, 190 Collings St, Clemson, SC 29634 USA;
6.Clemson Univ, Dept Forestry & Environm Conservat, 190 Collings St, Clemson, SC 29634 USA;
7.Univ Costa Rica, Inst Clodomiro Picado, Fac Microbiol, San Jose 11501, Costa Rica
推荐引用方式
GB/T 7714
Borja, Miguel,Neri-Castro, Edgar,Castaneda-Gaytan, Gamaliel,et al. Biological and Proteolytic Variation in the Venom of Crotalus scutulatus scutulatus from Mexico[J]. Universidad Nacional Autónoma de México,2018,10(1).
APA Borja, Miguel.,Neri-Castro, Edgar.,Castaneda-Gaytan, Gamaliel.,Strickland, Jason L..,Parkinson, Christopher L..,...&Perez-Morales, Rebeca.(2018).Biological and Proteolytic Variation in the Venom of Crotalus scutulatus scutulatus from Mexico.TOXINS,10(1).
MLA Borja, Miguel,et al."Biological and Proteolytic Variation in the Venom of Crotalus scutulatus scutulatus from Mexico".TOXINS 10.1(2018).
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