Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1038/s41598-018-25611-5 |
Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment | |
Jin, Wenwen1,3; Long, Yan4; Fu, Chunhua1; Zhang, Libin1; Xiang, Jun3; Wang, Baoshan2; Li, Maoteng1,3 | |
通讯作者 | Zhang, Libin ; Xiang, Jun |
来源期刊 | SCIENTIFIC REPORTS |
ISSN | 2045-2322 |
出版年 | 2018 |
卷号 | 8 |
英文摘要 | As a medicinal plant widely planted in southwest karst of China, the study of adaptation mechanisms of Lonicera confusa, especially to karst calcium-rich environment, can provide important theoretical basis for repairing desertification by genetic engineering. In this study, the Ca2+ imaging in the leaves of L. confusa was explored by LSCM (Laser Scanning Confocal Microscopy) and TEM (Transmission Electron Microscopy), which revealed that the calcium could be transported to gland, epidermal hair and stoma in the leaves of L. confusa in high-Ca2+ environment. In addition, we simulated the growth environment of L. confusa and identified DEGs (Differentially Expressed Genes) under different Ca2+ concentrations by RNA sequencing. Further analysis showed that these DEGs were assigned with some important biological processes. Furthermore, a complex protein-protein interaction network among DEGs in L. Confusa was constructed and some important regulatory genes and transcription factors were identified. Taken together, this study displayed the Ca2+ transport and the accumulation of Ca2+ channels and pools in L. Confusa with high-Ca2+ treatment. Moreover, RNA sequencing provided a global picture of differential gene expression patterns in L. Confusa with high-Ca2+ treatment, which will help to reveal the molecular mechanism of the adaptation of L. confusa to high-Ca2+ environment in the future. |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000431401700001 |
WOS关键词 | ARABIDOPSIS ; OXALATE ; CELLS ; ACCUMULATION ; TRANSPORTERS ; INCREASES ; PROTEINS ; CHANNELS ; DROUGHT ; DEFENSE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/213069 |
作者单位 | 1.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Hubei, Peoples R China; 2.Shandong Normal Univ, Coll Life Sci, Jinan 250000, Shandong, Peoples R China; 3.Huanggang Normal Univ, Hubei Collaborat Innovat Ctr Characterist Resourc, Hubei Key Lab Econ Forest Germplasm Improvement &, Huanggang 438000, Peoples R China; 4.Chinese Acad Agr Sci, Inst Biotechnol, Beijing 100081, Peoples R China |
推荐引用方式 GB/T 7714 | Jin, Wenwen,Long, Yan,Fu, Chunhua,et al. Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment[J],2018,8. |
APA | Jin, Wenwen.,Long, Yan.,Fu, Chunhua.,Zhang, Libin.,Xiang, Jun.,...&Li, Maoteng.(2018).Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment.SCIENTIFIC REPORTS,8. |
MLA | Jin, Wenwen,et al."Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment".SCIENTIFIC REPORTS 8(2018). |
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