Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/j.plaphy.2018.08.002 |
AmDREB2C, from Ammopiptanthus mongolicus, enhances abiotic stress tolerance and regulates fatty acid composition in transgenic Arabidopsis | |
Yin, Yumei; Jiang, Xiaoxu; Ren, Meiyan; Xue, Min; Nan, Dina; Wang, Zhilin; Xing, Yanping; Wang, Maoyan | |
通讯作者 | Wang, Maoyan |
来源期刊 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
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ISSN | 0981-9428 |
出版年 | 2018 |
卷号 | 130页码:517-528 |
英文摘要 | Dehydration-responsive element-binding (DREB) transcription factors (TFs) play a vital role in plant response to abiotic stresses. However, little is known about DREB TFs in plants adapted to harsh environments and in the formation of polyunsaturated fatty acids (PUFAs), a major membrane component closely associated with plant stress tolerance. Here, we characterized AmDREB2C in Ammopiptanthus mongolicus (Maxim. ex kom.) Cheng F., a desert evergreen broadleaf shrub with a high tolerance to harsh environments. AmDREB2C encodes a canonical DREB2-type TF, and the protein was localized in the nucleus. AmDREB2C had the highest expression levels in leaves of naturally growing shrubs in the wild during the winter season of a year of sampling. The expression was also induced by cold, heat and drought stresses in laboratory-cultured seedlings. Moreover, AmDREB2C was most abundantly expressed in young leaves and immature seeds rather than other tissues of the shrubs. Constitutive expression of AmDREB2C in Arabidopsis enhanced freezing, heat and drought tolerances of the transgenic plants, likely through inducing the expression of important stress-responsive genes. The transgene also increased the level of linolenic acid (C18:3), a major PUFA in most plant species, in leaves and seeds of the transgenic plants. Correspondingly, the transcription of FAD3, FAD7 and FADE, three genes encoding fatty acid desaturases (FADs) responsible for the production of C18:3, showed a differential up-regulation in these two organs. This study provides new insight into the underlying molecular mechanisms of A. mongolicus’ ability to endure harsh environments and DREB TF regulation of fatty acid denaturation. |
英文关键词 | Ammopiptanthus mongolicus DREB transcription factor Abiotic stress Polyunsaturated fatty acid Fatty acid desaturase |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000444789200051 |
WOS关键词 | RESPONSIVE GENE-EXPRESSION ; TRANSCRIPTION FACTOR DREB2A ; OMEGA-3 DESATURASE ; GENOME-WIDE ; PLANTS ; THALIANA ; DROUGHT ; RICE ; OVEREXPRESSION ; UNSATURATION |
WOS类目 | Plant Sciences |
WOS研究方向 | Plant Sciences |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/212218 |
作者单位 | Inner Mongolia Agr Univ, Coll Life Sci, 306 Zhaowuda St, Hohhot 010018, Peoples R China |
推荐引用方式 GB/T 7714 | Yin, Yumei,Jiang, Xiaoxu,Ren, Meiyan,et al. AmDREB2C, from Ammopiptanthus mongolicus, enhances abiotic stress tolerance and regulates fatty acid composition in transgenic Arabidopsis[J],2018,130:517-528. |
APA | Yin, Yumei.,Jiang, Xiaoxu.,Ren, Meiyan.,Xue, Min.,Nan, Dina.,...&Wang, Maoyan.(2018).AmDREB2C, from Ammopiptanthus mongolicus, enhances abiotic stress tolerance and regulates fatty acid composition in transgenic Arabidopsis.PLANT PHYSIOLOGY AND BIOCHEMISTRY,130,517-528. |
MLA | Yin, Yumei,et al."AmDREB2C, from Ammopiptanthus mongolicus, enhances abiotic stress tolerance and regulates fatty acid composition in transgenic Arabidopsis".PLANT PHYSIOLOGY AND BIOCHEMISTRY 130(2018):517-528. |
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