Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s11240-018-1404-y |
Cell culture establishment and regulation of two phenylethanoid glycosides accumulation in cell suspension culture of desert plant Cistanche tubulosa | |
Liu, Xiao1; Yan, Yaru1; Liu, Yuyu1; Mo, Ting1,3; Wang, Xiaohui1; Song, Yuelin1; Chen, Qingliang2; Zhao, Yunfang1; Shi, Shepo1; Tu, Pengfei1,2 | |
通讯作者 | Liu, Xiao ; Shi, Shepo ; Tu, Pengfei |
来源期刊 | PLANT CELL TISSUE AND ORGAN CULTURE |
ISSN | 0167-6857 |
EISSN | 1573-5044 |
出版年 | 2018 |
卷号 | 134期号:1页码:107-118 |
英文摘要 | Cistanche tubulosa is one of the most valuable desert medicinal plants, whose cell culture investigations have been rarely reported before. Phenylethanoid glycosides (PhGs) are its major components with a wide range of pharmacological activities. In this article, callus culture and cell suspension of C. tubulosa were established. Fleshy stems were found to be the most suitable explants for callus induction, and the optimal medium for induction was B5 solid medium supplemented with 0.8 g/L casein hydrolysate, 20 g/L sucrose, 2 mg/L naphthaleneacetic acid (NAA), and 1 mg/L 6-benzyladenine (6-BA). Based on qualitative and quantitative determination of two PhGs (echinacoside and acteoside) contents, the effects of carbon source concentration, precursor feeding, and elicitor treatments on cell growth and two PhGs accumulation in cell suspension cultures were investigated. Thirty g/L was the optimal initial sucrose concentration to obtain the high yield of biomass (9.29 g dry weight, DW) per liter cell suspension culture, echinacoside (12.14%, based on DW cells) and acteoside (2.17%). Precursor feeding also had a positive effect on PhGs accumulation. Feeding of precursor tyrosine (1 g/L) to the cell cultures increased the levels of echinacoside to 18.83% and acteoside to 2.92%, which were approximate 1.5 times of the corresponding levels in the control group. Methyl jasmonate (MJ) was the ideal elicitor for PhGs accumulations in C. tubulosa, particularly for eliciting acteoside production. The maximum echinacoside and acteoside contents reached 21.18 and 5.24% after 12 h of treatment with 200 A mu M MJ, respectively, which were approximate twofold higher than those in wild plant. |
英文关键词 | Cistanche tubulosa Callus induction Cell suspension culture Phenylethanoid glycosides |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000434910000009 |
WOS关键词 | MASS-SPECTROMETRY ; METHYL JASMONATE ; CALLUS-CULTURES ; SALICYLIC-ACID ; ELICITATION ; BIOSYNTHESIS ; ANTHOCYANIN ; TYROSINE ; DEFENSE ; GINSENG |
WOS类目 | Biotechnology & Applied Microbiology ; Plant Sciences |
WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
来源机构 | 北京大学 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/212184 |
作者单位 | 1.Beijing Univ Chinese Med, Sch Chinese Mat Med, Modern Res Ctr Tradit Chinese Med, Beijing 100029, Peoples R China; 2.Peking Univ, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China; 3.Ningxia Hui Med Res Inst, Yinchuan 750021, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Xiao,Yan, Yaru,Liu, Yuyu,et al. Cell culture establishment and regulation of two phenylethanoid glycosides accumulation in cell suspension culture of desert plant Cistanche tubulosa[J]. 北京大学,2018,134(1):107-118. |
APA | Liu, Xiao.,Yan, Yaru.,Liu, Yuyu.,Mo, Ting.,Wang, Xiaohui.,...&Tu, Pengfei.(2018).Cell culture establishment and regulation of two phenylethanoid glycosides accumulation in cell suspension culture of desert plant Cistanche tubulosa.PLANT CELL TISSUE AND ORGAN CULTURE,134(1),107-118. |
MLA | Liu, Xiao,et al."Cell culture establishment and regulation of two phenylethanoid glycosides accumulation in cell suspension culture of desert plant Cistanche tubulosa".PLANT CELL TISSUE AND ORGAN CULTURE 134.1(2018):107-118. |
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