Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s11816-018-0484-9 |
Micropropagation of the Mongolian medicinal plant Zygophyllum potaninii via somatic embryogenesis | |
Bayarmaa, Gun-Aajav1; Lee, Na Nyum3; Kang, Ho Duck2; Oyuntsetseg, Batlai1; Moon, Heung Kyu3 | |
通讯作者 | Moon, Heung Kyu |
来源期刊 | PLANT BIOTECHNOLOGY REPORTS
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ISSN | 1863-5466 |
EISSN | 1863-5474 |
出版年 | 2018 |
卷号 | 12期号:3页码:187-194 |
英文摘要 | The Mongolian medicinal plant Zygophyllum potaninii has been assessed as an endangered species with regional status. We applied the somatic embryogenesis technique using aseptic in vitro germinants of the plant as an effective propagation technology. The seed germination rate in vitro was 16.5% after 2 weeks of culture. Embryonic calli (EC) and somatic embryos (SEs) were induced using the cotyledon or hypocotyl segments of the germinants. Calli were effectively induced on MS medium supplemented with 0.1 mg/L 2, 4-dichlorophenoxy acetic acid (2, 4-d) and 0.5 mg/L 6-benzylamino purine (BA). The callus was composed of pale yellow or pale green friable cells. SE formed from EC only on Murashige and Skoog medium (MS) with 0.5 mg/L abscisic acid (ABA). Other concentrations of ABA failed to induce SE formation. All SEs germinated in MS medium with different salt levels. However, normal plant conversion was achieved only on half-strength MS medium. The converted plantlets were effectively acclimatized in vitro in sand and transferred to a mixture of sand and perlite (1:1 v/v) in the greenhouse. After 8 weeks of culture, 55.4% of the plants survived. This is a first report of propagating the medicinal desert plant Z. potaninii via somatic embryogenesis and plant regeneration. |
英文关键词 | Desert species Embryogenic cell Plant regeneration Sand bed acclimation |
类型 | Article |
语种 | 英语 |
国家 | Mongolia ; South Korea |
收录类别 | SCI-E |
WOS记录号 | WOS:000434386400004 |
WOS关键词 | MORPHOGENESIS ; MATURATION ; EXPLANTS ; CULTURES ; EMBRYOS |
WOS类目 | Biotechnology & Applied Microbiology ; Plant Sciences |
WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/212176 |
作者单位 | 1.Natl Univ Mongolia, Sch Arts & Sci, Div Nat Sci, Dept Biol, Ikh Surguuliin Gudamj 1, Sukhbaatar Dist, Ulaanbaatar, Mongolia; 2.Dongguk Univ, Dept Environm Sci & Ecol Engn, Coll Life Sci & Biotechnol, Lab Environm Biotechnol, 30,Pildong Ro 1 Gil, Seoul 04620, South Korea; 3.Natl Inst Forest Sci NIFoS, Div Forest Biotechnol, 39 Onjeong Ro, Suwon 16631, South Korea |
推荐引用方式 GB/T 7714 | Bayarmaa, Gun-Aajav,Lee, Na Nyum,Kang, Ho Duck,et al. Micropropagation of the Mongolian medicinal plant Zygophyllum potaninii via somatic embryogenesis[J],2018,12(3):187-194. |
APA | Bayarmaa, Gun-Aajav,Lee, Na Nyum,Kang, Ho Duck,Oyuntsetseg, Batlai,&Moon, Heung Kyu.(2018).Micropropagation of the Mongolian medicinal plant Zygophyllum potaninii via somatic embryogenesis.PLANT BIOTECHNOLOGY REPORTS,12(3),187-194. |
MLA | Bayarmaa, Gun-Aajav,et al."Micropropagation of the Mongolian medicinal plant Zygophyllum potaninii via somatic embryogenesis".PLANT BIOTECHNOLOGY REPORTS 12.3(2018):187-194. |
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