Arid
DOI10.1093/nar/gky797
Developmental and cancer-associated plasticity of DNA replication preferentially targets GC-poor, lowly expressed and late-replicating regions
Wu, Xia1,2; Kabalane, Hadi3; Kahli, Malik1,11; Petryk, Nataliya1,12; Laperrousaz, Bastien3,4; Jaszczyszyn, Yan5; Drillon, Guenola3; Nicolini, Frank-Emmanuel4,6; Perot, Gaelle7; Robert, Aude8; Fund, Cederic9; Chibon, Frederic7,13; Xia, Ruohong2; Wiels, Joelle8; Argoul, Francois10; Maguer-Satta, Veronique4; Arneodo, Alain10; Audit, Benjamin3; Hyrien, Olivier1
通讯作者Audit, Benjamin ; Hyrien, Olivier
来源期刊NUCLEIC ACIDS RESEARCH
ISSN0305-1048
EISSN1362-4962
出版年2018
卷号46期号:19页码:10157-10172
英文摘要

The spatiotemporal program of metazoan DNA replication is regulated during development and altered in cancers. We have generated novel OK-seq, Repliseq and RNA-seq data to compare the DNA replication and gene expression programs of twelve cancer and non-cancer human cell types. Changes in replication fork directionality (RFD) determined by OK-seq are widespread but more frequent within GC-poor isochores and largely disconnected from transcription changes. Cancer cell RFD profiles cluster with non-cancer cells of similar developmental origin but not with different cancer types. Importantly, recurrent RFD changes are detected in specific tumour progression pathways. Using a model for establishment and early progression of chronic myeloid leukemia (CML), we identify 1027 replication initiation zones (IZs) that progressively change efficiency during long-term expression of the BCR-ABL1 oncogene, being twice more often downregulated than upregulated. Prolonged expression of BCR-ABL1 results in targeting of new IZs and accentuation of previous efficiency changes. Targeted IZs are predominantly located in GC-poor, late replicating gene deserts and frequently silenced in late CML. Prolonged expression of BCR-ABL1 results in massive deletion of GC-poor, late replicating DNA sequences enriched in origin silencing events. We conclude that BCR-ABL1 expression progressively affects replication and stability of GC-poor, late-replicating regions during CML progression.


类型Article
语种英语
国家France ; Peoples R China ; USA ; Denmark
收录类别SCI-E
WOS记录号WOS:000450955800026
WOS关键词CHRONIC MYELOGENOUS LEUKEMIA ; DAMAGE-RESPONSE ; DOMAINS ; STRESS ; LANDSCAPE ; REVEALS ; BARRIER ; MODEL ; GENE ; PART
WOS类目Biochemistry & Molecular Biology
WOS研究方向Biochemistry & Molecular Biology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/211859
作者单位1.PSL Res Univ, CNRS, INSERM, Ecole Normale Super,Dept Biol,IBENS, F-75005 Paris, France;
2.East China Normal Univ, Phys Dept, Shanghai, Peoples R China;
3.Univ Claude Bernard Lyon 1, Univ Lyon, CNRS, Lab Phys,ENS Lyon, F-69342 Lyon, France;
4.Ctr Rech & Cancerol Lyon, CNRS, INSERM, UMR5286,U1052, F-69008 Lyon, France;
5.Univ Paris Saclay, Univ Paris Sud, CNRS, CEA,Inst Integrat Biol Cell I2BC, Gif Sur Yvette, France;
6.Ctr Leon Berard, F-69008 Lyon, France;
7.INSERM, U1218, Inst Bergonie, F-33000 Bordeaux, France;
8.Univ Paris Sud Paris Saclay, CNRS, Inst Gustave Roussy, UMR 8126, Villejuif, France;
9.PSL Res Univ, CNRS, INSERM, IBENS,Plateforme Genom,Ecole Normale Super, F-75005 Paris, France;
10.Univ Bordeaux, CNRS, LOMA, UMR 5798, F-33405 Talence, France;
11.NYU, Dept Biol, New York, NY 10003 USA;
12.Univ Copenhagen, BRIC, Ole Maaloes Vej 5, DK-2200 Copenhagen N, Denmark;
13.CRCT, INSERM, U1037, Inst Claudius Regaud, F-31037 Toulouse, France
推荐引用方式
GB/T 7714
Wu, Xia,Kabalane, Hadi,Kahli, Malik,et al. Developmental and cancer-associated plasticity of DNA replication preferentially targets GC-poor, lowly expressed and late-replicating regions[J],2018,46(19):10157-10172.
APA Wu, Xia.,Kabalane, Hadi.,Kahli, Malik.,Petryk, Nataliya.,Laperrousaz, Bastien.,...&Hyrien, Olivier.(2018).Developmental and cancer-associated plasticity of DNA replication preferentially targets GC-poor, lowly expressed and late-replicating regions.NUCLEIC ACIDS RESEARCH,46(19),10157-10172.
MLA Wu, Xia,et al."Developmental and cancer-associated plasticity of DNA replication preferentially targets GC-poor, lowly expressed and late-replicating regions".NUCLEIC ACIDS RESEARCH 46.19(2018):10157-10172.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wu, Xia]的文章
[Kabalane, Hadi]的文章
[Kahli, Malik]的文章
百度学术
百度学术中相似的文章
[Wu, Xia]的文章
[Kabalane, Hadi]的文章
[Kahli, Malik]的文章
必应学术
必应学术中相似的文章
[Wu, Xia]的文章
[Kabalane, Hadi]的文章
[Kahli, Malik]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。