Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s00572-018-0867-3 |
Mycelium of Terfezia claveryi as inoculum source to produce desert truffle mycorrhizal plants | |
Arenas, Francisco1; Navarro-Rodenas, Alfonso1; Chavez, Daniel2; Gutierrez, Almudena1; Perez-Gilabert, Manuela3; Morte, Asuncion1 | |
通讯作者 | Morte, Asuncion |
来源期刊 | MYCORRHIZA
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ISSN | 0940-6360 |
EISSN | 1432-1890 |
出版年 | 2018 |
卷号 | 28期号:7页码:691-701 |
英文摘要 | Terfezia claveryi Chatin was the first desert truffle species to be cultivated, the mycorrhizal plants being successfully produced by using both desert truffle spores and mycelia. However, it is more advisable to use mycelium than spores whenever possible and profitable. Given the low yields of mycelia obtained using traditional culture methods of this truffle, the medium composition was modified in an attempt to determine its nutritional requirements. For this, an assay involving response surface methodology was performed using Box-Bchnken design to find the optimal parameters for the high production of mycelial biomass. The best results were obtained with glucose as carbon source, buffering the pH at 5 during culture, adding a pool of vitamins, and adjusting the optimal concentrations of carbon and nitrogen sources of the MMN medium. Biomass production increased from 0.3 to 3 g L-1 dry weight and productivity increased from 10.7 to 95.8 mg L(-1 )day(-1) dry weight. The produced mycelium was able to colonize Helianthemum roots efficiently, providing more than 50% ectomycorrhizal colonization. |
英文关键词 | Mycelial biomass Box-Behnken design Response surface methodology Desert truffle Terfezia claveryi |
类型 | Article |
语种 | 英语 |
国家 | Spain ; Chile |
收录类别 | SCI-E |
WOS记录号 | WOS:000447241100009 |
WOS关键词 | FUNGUS PISOLITHUS-MICROCARPUS ; ECTOMYCORRHIZAL FUNGI ; TUBER POLYSACCHARIDES ; SUBMERGED CULTIVATION ; OPTIMIZATION ; GROWTH ; ROOTS ; MELANOSPORUM ; FERMENTATION ; BIOREACTOR |
WOS类目 | Mycology |
WOS研究方向 | Mycology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/211748 |
作者单位 | 1.Univ Murcia, Dept Biol Vegetal, Fac Biol, Campus Espinardo, E-30100 Murcia, Spain; 2.Univ Concepcion, Dept Ciencias & Tecnol Vegetal, Escuela Ciencias & Tecnol, Campus Los Angeles, Los Angeles, Chile; 3.Univ Murcia, Dept Bioquim & Biol Mol A, Fac Vet, Campues Espinardo, E-30100 Murcia, Spain |
推荐引用方式 GB/T 7714 | Arenas, Francisco,Navarro-Rodenas, Alfonso,Chavez, Daniel,et al. Mycelium of Terfezia claveryi as inoculum source to produce desert truffle mycorrhizal plants[J],2018,28(7):691-701. |
APA | Arenas, Francisco,Navarro-Rodenas, Alfonso,Chavez, Daniel,Gutierrez, Almudena,Perez-Gilabert, Manuela,&Morte, Asuncion.(2018).Mycelium of Terfezia claveryi as inoculum source to produce desert truffle mycorrhizal plants.MYCORRHIZA,28(7),691-701. |
MLA | Arenas, Francisco,et al."Mycelium of Terfezia claveryi as inoculum source to produce desert truffle mycorrhizal plants".MYCORRHIZA 28.7(2018):691-701. |
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