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DOI | 10.1134/S0026261718040069 |
Aminopeptidase Activity of Haloalkalophilic Bacteria of the Genus Halomonas Isolated from the Soda-Saline Lakes in the Badain Jaran Desert | |
Erdyneeva, E. B.1; Radnagurueva, A. A.1; Dunaevsky, Ya. E.2; Belkova, N. L.3; Namsaraev, Z. B.4; Lavrentieva, E. V.1,5 | |
通讯作者 | Lavrentieva, E. V. |
来源期刊 | MICROBIOLOGY |
ISSN | 0026-2617 |
EISSN | 1608-3237 |
出版年 | 2018 |
卷号 | 87期号:4页码:538-548 |
英文摘要 | Three strains of haloalkaliphilic bacteria were isolated from microbial mats of soda-saline lakes of the Badain Jaran Desert, Inner Mongolia (China). Based on the data on ribosomal phylogeny, they were identified as members of the genus Halomonas. These bacteria were moderate alkaliphiles and extreme halophiles. The peptidases secreted by these bacteria were shown to have narrow substrate specificity. They hydrolyzed proteins and para-nitroanilide substrates and showed maximal activity in the hydrolysis of L-leucyl-p-nitroanilides (LpNA). The maximum activity of the peptidases occurred at alkaline pH values (8-10) and elevated salinity (50-100 g/L); the enzymes were thermostable (up to 50A degrees D). The results of inhibitor analysis and substrate specificity examination of extracellular enzymes indicated them to belong to the class of aminopeptidase-like metallopeptidases. |
英文关键词 | Halomonas aminopeptidases Badain Jaran Desert saline soda lakes |
类型 | Article |
语种 | 英语 |
国家 | Russia |
收录类别 | SCI-E |
WOS记录号 | WOS:000438825500011 |
WOS关键词 | BACILLUS-LICHENIFORMIS SWJS33 ; SP NOV. ; HALOPHILIC BACTERIA ; PROTEIN ISOLATE ; PURIFICATION ; HYDROLYSIS ; ENZYMES ; SALT |
WOS类目 | Microbiology |
WOS研究方向 | Microbiology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/211658 |
作者单位 | 1.Russian Acad Sci, Inst Gen & Expt Biol, Siberian Branch, Ulan Ude, Russia; 2.Lomonosov Moscow State Univ, Moscow, Russia; 3.Russian Acad Sci, Limnol Inst, Siberian Branch, Irkutsk, Russia; 4.Kurchatov Inst, Moscow, Russia; 5.Buryat State Univ, Ulan Ude, Russia |
推荐引用方式 GB/T 7714 | Erdyneeva, E. B.,Radnagurueva, A. A.,Dunaevsky, Ya. E.,et al. Aminopeptidase Activity of Haloalkalophilic Bacteria of the Genus Halomonas Isolated from the Soda-Saline Lakes in the Badain Jaran Desert[J],2018,87(4):538-548. |
APA | Erdyneeva, E. B.,Radnagurueva, A. A.,Dunaevsky, Ya. E.,Belkova, N. L.,Namsaraev, Z. B.,&Lavrentieva, E. V..(2018).Aminopeptidase Activity of Haloalkalophilic Bacteria of the Genus Halomonas Isolated from the Soda-Saline Lakes in the Badain Jaran Desert.MICROBIOLOGY,87(4),538-548. |
MLA | Erdyneeva, E. B.,et al."Aminopeptidase Activity of Haloalkalophilic Bacteria of the Genus Halomonas Isolated from the Soda-Saline Lakes in the Badain Jaran Desert".MICROBIOLOGY 87.4(2018):538-548. |
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