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DOI | 10.1016/j.jpba.2018.01.019 |
Differential mobility spectrometry followed by tandem mass spectrometry with multiple ion monitoring for bioanalysis of eptifibatide in rat plasma | |
Ren, Tianming1,2; Li, Runzhi1,2; Meng, Xiangjun1,2; Fawcett, J. Paul3; Sun, Dong4; Gu, Jingkai2 | |
通讯作者 | Sun, Dong ; Gu, Jingkai |
来源期刊 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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ISSN | 0731-7085 |
EISSN | 1873-264X |
出版年 | 2018 |
卷号 | 151页码:260-265 |
英文摘要 | Eptifibatide is a therapeutic cyclic peptide with poor collision-induced dissociation (CID) efficiency for multiple reaction monitoring (MRM), which limits the development of a traditional liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioassay with MRM. In this study, a method combining differential mobility spectrometry (DMS) with liquid chromatography-multiple ion monitoring (LC-DMS-MIM) was developed for the quantitation of eptifibatide in rat plasma. After solid phase extraction (SPE) of 100 mu L plasma on an Oasis (R) HLB cartridge, the analyte and I.S. (octreotide) were analyzed using a SCIEX QTRAP 6500 operated in the positive ion mode and preceded by a DMS device. The lower limit of quantitation (LLOQ) for eptifibatide was 0.5 ng/mL using only 100 mu L plasma. The method was linear in the concentration range 0.5-300 ng/mL with good precision and accuracy. Compared to regulated quantitative LC-MS/MS bioanalysis of eptifibatide, the LC-DMS-MIM method effectively overcomes the sensitivity challenge in the LC-MRM method and reduces the high background noise and matrix interference in LC-MIM method without DMS. The method was successfully applied to a pharmacokinetic study involving intravenous injection of eptifibatide to Wistar rats. (C) 2018 Elsevier B.V. All rights reserved. |
英文关键词 | Eptifibatide Differential mobility spectrometry Multiple ion monitoring Poor collision-induced dissociation efficiency |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China ; New Zealand |
收录类别 | SCI-E |
WOS记录号 | WOS:000426025700031 |
WOS类目 | Chemistry, Analytical ; Pharmacology & Pharmacy |
WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/211220 |
作者单位 | 1.Jilin Univ, Sch Life Sci, Changchun 130012, Jilin, Peoples R China; 2.Jilin Univ, Res Ctr Drug Metab, Changchun 130012, Jilin, Peoples R China; 3.Univ Otago, Sch Pharm, POB 56, Dunedin, New Zealand; 4.Beijing Inst Modern Drug Metab, Zhengping Rd, Beijing 102209, Peoples R China |
推荐引用方式 GB/T 7714 | Ren, Tianming,Li, Runzhi,Meng, Xiangjun,et al. Differential mobility spectrometry followed by tandem mass spectrometry with multiple ion monitoring for bioanalysis of eptifibatide in rat plasma[J],2018,151:260-265. |
APA | Ren, Tianming,Li, Runzhi,Meng, Xiangjun,Fawcett, J. Paul,Sun, Dong,&Gu, Jingkai.(2018).Differential mobility spectrometry followed by tandem mass spectrometry with multiple ion monitoring for bioanalysis of eptifibatide in rat plasma.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,151,260-265. |
MLA | Ren, Tianming,et al."Differential mobility spectrometry followed by tandem mass spectrometry with multiple ion monitoring for bioanalysis of eptifibatide in rat plasma".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 151(2018):260-265. |
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