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DOI10.1016/j.jpba.2018.01.019
Differential mobility spectrometry followed by tandem mass spectrometry with multiple ion monitoring for bioanalysis of eptifibatide in rat plasma
Ren, Tianming1,2; Li, Runzhi1,2; Meng, Xiangjun1,2; Fawcett, J. Paul3; Sun, Dong4; Gu, Jingkai2
通讯作者Sun, Dong ; Gu, Jingkai
来源期刊JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN0731-7085
EISSN1873-264X
出版年2018
卷号151页码:260-265
英文摘要

Eptifibatide is a therapeutic cyclic peptide with poor collision-induced dissociation (CID) efficiency for multiple reaction monitoring (MRM), which limits the development of a traditional liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioassay with MRM. In this study, a method combining differential mobility spectrometry (DMS) with liquid chromatography-multiple ion monitoring (LC-DMS-MIM) was developed for the quantitation of eptifibatide in rat plasma. After solid phase extraction (SPE) of 100 mu L plasma on an Oasis (R) HLB cartridge, the analyte and I.S. (octreotide) were analyzed using a SCIEX QTRAP 6500 operated in the positive ion mode and preceded by a DMS device. The lower limit of quantitation (LLOQ) for eptifibatide was 0.5 ng/mL using only 100 mu L plasma. The method was linear in the concentration range 0.5-300 ng/mL with good precision and accuracy. Compared to regulated quantitative LC-MS/MS bioanalysis of eptifibatide, the LC-DMS-MIM method effectively overcomes the sensitivity challenge in the LC-MRM method and reduces the high background noise and matrix interference in LC-MIM method without DMS. The method was successfully applied to a pharmacokinetic study involving intravenous injection of eptifibatide to Wistar rats. (C) 2018 Elsevier B.V. All rights reserved.


英文关键词Eptifibatide Differential mobility spectrometry Multiple ion monitoring Poor collision-induced dissociation efficiency
类型Article
语种英语
国家Peoples R China ; New Zealand
收录类别SCI-E
WOS记录号WOS:000426025700031
WOS类目Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向Chemistry ; Pharmacology & Pharmacy
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/211220
作者单位1.Jilin Univ, Sch Life Sci, Changchun 130012, Jilin, Peoples R China;
2.Jilin Univ, Res Ctr Drug Metab, Changchun 130012, Jilin, Peoples R China;
3.Univ Otago, Sch Pharm, POB 56, Dunedin, New Zealand;
4.Beijing Inst Modern Drug Metab, Zhengping Rd, Beijing 102209, Peoples R China
推荐引用方式
GB/T 7714
Ren, Tianming,Li, Runzhi,Meng, Xiangjun,et al. Differential mobility spectrometry followed by tandem mass spectrometry with multiple ion monitoring for bioanalysis of eptifibatide in rat plasma[J],2018,151:260-265.
APA Ren, Tianming,Li, Runzhi,Meng, Xiangjun,Fawcett, J. Paul,Sun, Dong,&Gu, Jingkai.(2018).Differential mobility spectrometry followed by tandem mass spectrometry with multiple ion monitoring for bioanalysis of eptifibatide in rat plasma.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,151,260-265.
MLA Ren, Tianming,et al."Differential mobility spectrometry followed by tandem mass spectrometry with multiple ion monitoring for bioanalysis of eptifibatide in rat plasma".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 151(2018):260-265.
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