Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s12275-018-8176-2 |
Identification of trehalose as a compatible solute in different species of acidophilic bacteria | |
Galleguillos, Pedro A.1,2; Grail, Barry M.3; Hallberg, Kevin B.3; Demergasso, Cecilia S.1,2; Johnson, D. Barrie3 | |
通讯作者 | Galleguillos, Pedro A. |
来源期刊 | JOURNAL OF MICROBIOLOGY
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ISSN | 1225-8873 |
EISSN | 1976-3794 |
出版年 | 2018 |
卷号 | 56期号:10页码:727-733 |
英文摘要 | The major industrial heap bioleaching processes are located in desert regions (mainly Chile and Australia) where fresh water is scarce and the use of resources with low water activity becomes an attractive alternative. However, in spite of the importance of the microbial populations involved in these processes, little is known about their response or adaptation to osmotic stress. In order to investigate the response to osmotic stress in these microorganisms, six species of acidophilic bacteria were grown at elevated osmotic strength in liquid media, and the compatible solutes synthesised were identified using ion chromatography and MALDI-TOF mass spectrometry. Trehalose was identified as one of, or the sole, compatible solute in all species and strains, apart from Acidithiobacillus thiooxidans where glucose and proline levels increased at elevated osmotic potentials. Several other potential compatible solutes were tentatively identified by MALDITOF analysis. The same compatible solutes were produced by these bacteria regardless of the salt used to produce the osmotic stress. The results correlate with data from sequenced genomes which confirm that many chemolithotrophic and heterotrophic acidophiles possess genes for trehalose synthesis. This is the first report to identify and quantify compatible solutes in acidophilic bacteria that have important roles in biomining technologies. |
英文关键词 | bioleaching acidophiles compatible solutes trehalose sulfate osmolytes |
类型 | Article |
语种 | 英语 |
国家 | Chile ; Wales |
收录类别 | SCI-E |
WOS记录号 | WOS:000446020000005 |
WOS关键词 | ACID-MINE DRAINAGE ; RHODOTHERMUS-MARINUS ; GLYCINE BETAINE ; IRON MOUNTAIN ; SP-NOV ; LEPTOSPIRILLUM ; METABOLISM ; ACCUMULATION ; CALIFORNIA ; COMMUNITY |
WOS类目 | Microbiology |
WOS研究方向 | Microbiology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/211170 |
作者单位 | 1.Sci & Technol Res Ctr Min CICITEM, Sucre 220,602, Antofagasta, Chile; 2.Univ Catolica Norte, Biotechnol Ctr, Avda Angamos 0610, Antofagasta, Chile; 3.Bangor Univ, Coll Nat Sci, Bangor LL57 2UW, Gwynedd, Wales |
推荐引用方式 GB/T 7714 | Galleguillos, Pedro A.,Grail, Barry M.,Hallberg, Kevin B.,et al. Identification of trehalose as a compatible solute in different species of acidophilic bacteria[J],2018,56(10):727-733. |
APA | Galleguillos, Pedro A.,Grail, Barry M.,Hallberg, Kevin B.,Demergasso, Cecilia S.,&Johnson, D. Barrie.(2018).Identification of trehalose as a compatible solute in different species of acidophilic bacteria.JOURNAL OF MICROBIOLOGY,56(10),727-733. |
MLA | Galleguillos, Pedro A.,et al."Identification of trehalose as a compatible solute in different species of acidophilic bacteria".JOURNAL OF MICROBIOLOGY 56.10(2018):727-733. |
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