Knowledge Resource Center for Ecological Environment in Arid Area
Production of Recombinant Protein of Salsola Kali (Sal k1) Pollen Allergen in Lactococcus Lactis | |
Ghasemi, Ziba1; Varasteh, Abdol-Reza2; Moghadam, Malihe1; Sedghi, Farnaz1; Jabbariazad, Farahzad2; Sankian, Mojtaba1 | |
通讯作者 | Sankian, Mojtaba |
来源期刊 | IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY
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ISSN | 1735-1502 |
EISSN | 1735-5249 |
出版年 | 2018 |
卷号 | 17期号:2页码:134-143 |
英文摘要 | The Salsola kali pollen is considered the main cause of allergic sensitization in desert and semi-desert regions. We have constructed recombinant luxtococcus lactis producing Sal k1 protein with the aim of using it as a mucosal vaccine for specific immunotherapy. The Sal k1 gene was amplified, and transferred into a PNZ 8148 plasmid. The PNZ8148-Sal k1 recombinant plasmid was transformed into competent E.coli strain MC1061 for replication, and then was isolated and cloned into competent L. lactis by electroporation. The cloning was verified by PCR and gene sequencing. The production of recombinant Sal K1 (rSal K1) protein was induced by nisin. The rSal K1 protein was purified by affinity chromatography and dialysis, and confirmed by SDS-PAGE and western blot analyses. The recombinant L. lactis was successfully constructed. Production of a 40-kDa rSal k1 protein with the L. lactis was shown by sodium dodecyl sulfate-polyacrylamid gel electrophoresis (SDS-PAGE) analysis. In addition, western blot analysis using specific mouse anti-Sal k1 polyclonal antibodies and sensitive human sera verified the 40-kD protein as rSal k1 allergen. This study demonstrated that L. lactis may be used as a promising live delivery system for recombinant Sal k1 protein without altering its immunoreactivity; however, its efficacy in the context of the immune system is suggested to be pursued in future studies. |
英文关键词 | Lactococcus lactis Pollen Recombinant protein Salsola kali Sal k1 |
类型 | Article |
语种 | 英语 |
国家 | Iran |
收录类别 | SCI-E |
WOS记录号 | WOS:000432327700004 |
WOS关键词 | BOVINE BETA-LACTOGLOBULIN ; PRICK TEST REACTIVITY ; ACID BACTERIA ; THERAPEUTIC PROTEINS ; COMMON AEROALLERGENS ; ATOPIC-DERMATITIS ; IMMUNE-RESPONSES ; PROBIOTICS ; DELIVERY ; MICE |
WOS类目 | Allergy ; Immunology |
WOS研究方向 | Allergy ; Immunology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/210317 |
作者单位 | 1.Mashhad Univ Med Sci, Immunol Res Ctr, Sch Med, Mashhad, Iran; 2.Mashhad Univ Med Sci, Allergy Res Ctr, Mashhad, Iran |
推荐引用方式 GB/T 7714 | Ghasemi, Ziba,Varasteh, Abdol-Reza,Moghadam, Malihe,et al. Production of Recombinant Protein of Salsola Kali (Sal k1) Pollen Allergen in Lactococcus Lactis[J],2018,17(2):134-143. |
APA | Ghasemi, Ziba,Varasteh, Abdol-Reza,Moghadam, Malihe,Sedghi, Farnaz,Jabbariazad, Farahzad,&Sankian, Mojtaba.(2018).Production of Recombinant Protein of Salsola Kali (Sal k1) Pollen Allergen in Lactococcus Lactis.IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY,17(2),134-143. |
MLA | Ghasemi, Ziba,et al."Production of Recombinant Protein of Salsola Kali (Sal k1) Pollen Allergen in Lactococcus Lactis".IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY 17.2(2018):134-143. |
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