干旱区生态环境知识资源中心

A Gram-stain-negative, aerobic, motile and rod-shaped bacterium, strain ZMN-3(T), was isolated from desert soil sample collected from Ongniod Qi, Inner Mongolia, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZMN-3(T) was affiliated with the genus Massilia and showed the highest similarity to Massilia humi THG S6-8(T) (98.9 %) and Massilia buxea A9(T) (98.2 %). In partial gyrB and lepA sequences, the highest similarity of strain ZMN-3(T) and M. humi THG S6-8(T) were 95.9 and 96.8 %, respectively. The DNA-DNA hybridization value between strain ZMN-3(T) and its closely related type strains were all below 70 %. The major respiratory quinone of strain ZMN-3(T) was Q-8 and the major cellular fatty acids consisted of summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) and C-16:0. The predominant polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content of strain ZMN-3(T) was 66.3 mol%. On the basis of this polyphasic taxonomic study, strain ZMN-3(T) is considered to represent a novel species of the genus Massilia, for which the name Massilia armeniaca sp. nov. is proposed. The type strain is ZMN-3(T) (=CGMCC 1.16209(T) = DSM 104676(T)).