Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.3390/ijms19020542 |
Analysis of Peptide Ligand Specificity of Different Insect Adipokinetic Hormone Receptors | |
Marchal, Elisabeth1; Schellens, Sam1; Monjon, Emilie1; Bruyninckx, Evert1; Marco, Heather G.2; Gade, Gerd2; Vanden Broeck, Jozef1; Verlinden, Heleen1 | |
通讯作者 | Verlinden, Heleen |
来源期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
ISSN | 1422-0067 |
出版年 | 2018 |
卷号 | 19期号:2 |
英文摘要 | Adipokinetic hormone (AKH) is a highly researched insect neuropeptide that induces the mobilization of carbohydrates and lipids from the fat body at times of high physical activity, such as flight and locomotion. As a naturally occurring ligand, AKH has undergone quite a number of amino acid changes throughout evolution, and in some insect species multiple AKHs are present. AKH acts by binding to a rhodopsin-like G protein-coupled receptor, which is related to the vertebrate gonadotropin-releasing hormone receptors. In the current study, we have cloned AKH receptors (AKHRs) from seven different species, covering a wide phylogenetic range of insect orders: the fruit fly, Drosophila melanogaster, and the yellow fever mosquito, Aedes aegypti (Diptera); the red flour beetle, Tribolium castaneum, and the large pine weevil, Hylobius abietis (Coleoptera); the honeybee, Apis mellifera (Hymenoptera); the pea aphid, Acyrthosiphon pisum (Hemiptera); and the desert locust, Schistocerca gregaria (Orthoptera). The agonistic activity of different insect AKHs, including the respective endogenous AKHs, at these receptors was tested with a bioluminescence-based assay in Chinese hamster ovary cells. All receptors were activated by their endogenous ligand in the nanomolar range. Based on our data, we can refute the previously formulated hypothesis that a functional AKH signaling system is absent in the beneficial species, Apis mellifera. Furthermore, our data also suggest that some of the investigated AKH receptors, such as the mosquito AKHR, are more selective for the endogenous (conspecific) ligand, while others, such as the locust AKHR, are more promiscuous and can be activated by AKHs from many other insects. This information will be of high importance when further analyzing the potential use of AKHRs as targets for developing novel pest control agents. |
英文关键词 | AKH carbohydrate energy GPCR lipid metabolism neuropeptide pest control |
类型 | Article |
语种 | 英语 |
国家 | Belgium ; South Africa |
收录类别 | SCI-E |
WOS记录号 | WOS:000427527400226 |
WOS关键词 | PROTEIN-COUPLED RECEPTORS ; DROSOPHILA-MELANOGASTER ; OXIDATIVE STRESS ; SIGNALING SYSTEM ; RHODNIUS-PROLIXUS ; APIS-MELLIFERA ; HONEY-BEE ; IDENTIFICATION ; NEUROPEPTIDES ; PHYSIOLOGY |
WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/210227 |
作者单位 | 1.Katholieke Univ Leuven, Mol Dev Physiol & Signal Transduct, Naamsestr 59,POB 02465, B-3000 Leuven, Belgium; 2.Univ Cape Town, Dept Biol Sci, ZA-7700 Rondebosch, South Africa |
推荐引用方式 GB/T 7714 | Marchal, Elisabeth,Schellens, Sam,Monjon, Emilie,et al. Analysis of Peptide Ligand Specificity of Different Insect Adipokinetic Hormone Receptors[J],2018,19(2). |
APA | Marchal, Elisabeth.,Schellens, Sam.,Monjon, Emilie.,Bruyninckx, Evert.,Marco, Heather G..,...&Verlinden, Heleen.(2018).Analysis of Peptide Ligand Specificity of Different Insect Adipokinetic Hormone Receptors.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(2). |
MLA | Marchal, Elisabeth,et al."Analysis of Peptide Ligand Specificity of Different Insect Adipokinetic Hormone Receptors".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.2(2018). |
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