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Comparative resistance status against synthetic pyrethroids (deltamethrin and cypermethrin) in two economically important ticks, Hyalomma anatolicum and Rhipicephalus (Boophilus) microplus, of North Gujarat was tested using commercially available deltamethrin (1.25%) and cypermethrin (10%) by Larval Packet Test. Results revealed that low level of resistance has been developed in H. anatolicum tick isolates of Vasda, Shihori, and Palanpur against deltamethrin (level I; RF = 2.39-3.79) and that for cypermethrin Vasda, Vav, Soneth, Palanpur tick isolates were found to be resistant (level I; RF = 1.5-4.6). The R. (B.) microplus isolates from Vav revealed highest resistance level IV (RF = 43.8) and level I resistance (RF = 1.77-3.52) from Mahadevpura, Vasda, Rooppura, Bhadath against deltamethrin. Resistance level for cypermethrin in Ranpur isolate was I (RF = 1.62); however, in Mahadevpura and Vasda tick isolateit was up to level II (RF = 17.26 and 8.16, respectively). R. (B.) microplus tick isolates from Vasda and Mahadevpura were resistant against both deltamethrin and cypermethrin with resistance level I and II, respectively. Similarly, H. anatolicum tick isolates from Vasda were resistant to both deltamethrin and cypermethrin with level I resistance (RF = 3.79 for deltamethrin and 1.8 for cypermethrin).