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Induction of high frequency somatic embryogenesis and analysis of developmental stagewise expression of SERK1 gene during somatic embryogenesis in cultures of Vigna radiata (L.) R.Wilczek | |
Sindhujaa, Vajravel1; Gnanaraj, Muniraj1; Viji, Maluventhen2; Karuppanapandian, Thirupathi3; Manoharan, Kumariah1 | |
通讯作者 | Manoharan, Kumariah |
来源期刊 | INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY
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ISSN | 0019-5189 |
EISSN | 0975-1009 |
出版年 | 2018 |
卷号 | 56期号:3页码:180-193 |
英文摘要 | Vigna radiata (L.) R.Wilczek (Fabaceae), commonly called Green gram or Mung bean, is an important legume with potential nutritional, medicinal and health benefits cultivated widespread throughout the rain-fed areas of arid and semi-arid tropics and subtropics. Being an affordable source of carbohydrate, vitamins, minerals and phytonutrients besides protein, Green gram finds demand for its nutrient digestibility, food processing properties and bioavailability. Though India ranks top in world mung bean production (>50%), it is unable to meet the local demand. Biotic and abiotic stresses restrict mung bean yield considerably and researchers have been working on resistant varieties to overcome these challenges. In this study, towards improving yield, an effective protocol for attaining high frequency somatic embryogenesis (SE) in green gram has been proposed. Type of explants and age of source seedlings for obtaining explants were found to influence the formation of embryogenic calli. Various combinations and concentrations of 2,4-dichlorophenoxyacetic acid and indole-3-acetic acid with kinetin were optimized for developing embryogenic calli. Embryogenic calli when exposed to osmotic stress created by D-mannitol and sorbitol and dehydration stress imposed by polyethylene glycol were found to produce somatic embryos. Calli incubated for 6 h in specified hormone free nutrient medium supplemented with 4% polyethylene glycol was optimal for induction of high frequency SE. Subsequent to stress incubation, the cultures formed only early stage somatic embryos. Supplementation of proline was found essential for the maturation of somatic embryos. Cotyledonary stage somatic embryos were converted into plantlets and subsequently established in garden soil. Semi-quantitative Reverse Transcription-PCR based transcript level analysis of SERK1 gene expression was carried out during different developmental stages of somatic embryogenesis. Expression of SERK1 was specifically associated with the embryogenic calli and calli enriched with globular stage somatic embryos. |
英文关键词 | Embryogenic competence Green gram Mung bean Proline Polyethylene glycol |
类型 | Article |
语种 | 英语 |
国家 | India ; Czech Republic |
收录类别 | SCI-E |
WOS记录号 | WOS:000427449400005 |
WOS关键词 | PLANT-REGENERATION ; ARABIDOPSIS ; LEGUMES ; LIFE |
WOS类目 | Biology |
WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/210018 |
作者单位 | 1.Madurai Kamaraj Univ, Sch Biol Sci, Dept Plant Morphol & Algol, Madurai 625021, Tamil Nadu, India; 2.Thiagarajar Coll, Dept Bot, Madurai 625009, Tamil Nadu, India; 3.Masaryk Univ, Fac Sci, Dept Expt Biol, Brno 62500, Czech Republic |
推荐引用方式 GB/T 7714 | Sindhujaa, Vajravel,Gnanaraj, Muniraj,Viji, Maluventhen,et al. Induction of high frequency somatic embryogenesis and analysis of developmental stagewise expression of SERK1 gene during somatic embryogenesis in cultures of Vigna radiata (L.) R.Wilczek[J],2018,56(3):180-193. |
APA | Sindhujaa, Vajravel,Gnanaraj, Muniraj,Viji, Maluventhen,Karuppanapandian, Thirupathi,&Manoharan, Kumariah.(2018).Induction of high frequency somatic embryogenesis and analysis of developmental stagewise expression of SERK1 gene during somatic embryogenesis in cultures of Vigna radiata (L.) R.Wilczek.INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY,56(3),180-193. |
MLA | Sindhujaa, Vajravel,et al."Induction of high frequency somatic embryogenesis and analysis of developmental stagewise expression of SERK1 gene during somatic embryogenesis in cultures of Vigna radiata (L.) R.Wilczek".INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY 56.3(2018):180-193. |
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