Arid
Induction of high frequency somatic embryogenesis and analysis of developmental stagewise expression of SERK1 gene during somatic embryogenesis in cultures of Vigna radiata (L.) R.Wilczek
Sindhujaa, Vajravel1; Gnanaraj, Muniraj1; Viji, Maluventhen2; Karuppanapandian, Thirupathi3; Manoharan, Kumariah1
通讯作者Manoharan, Kumariah
来源期刊INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY
ISSN0019-5189
EISSN0975-1009
出版年2018
卷号56期号:3页码:180-193
英文摘要

Vigna radiata (L.) R.Wilczek (Fabaceae), commonly called Green gram or Mung bean, is an important legume with potential nutritional, medicinal and health benefits cultivated widespread throughout the rain-fed areas of arid and semi-arid tropics and subtropics. Being an affordable source of carbohydrate, vitamins, minerals and phytonutrients besides protein, Green gram finds demand for its nutrient digestibility, food processing properties and bioavailability. Though India ranks top in world mung bean production (>50%), it is unable to meet the local demand. Biotic and abiotic stresses restrict mung bean yield considerably and researchers have been working on resistant varieties to overcome these challenges. In this study, towards improving yield, an effective protocol for attaining high frequency somatic embryogenesis (SE) in green gram has been proposed. Type of explants and age of source seedlings for obtaining explants were found to influence the formation of embryogenic calli. Various combinations and concentrations of 2,4-dichlorophenoxyacetic acid and indole-3-acetic acid with kinetin were optimized for developing embryogenic calli. Embryogenic calli when exposed to osmotic stress created by D-mannitol and sorbitol and dehydration stress imposed by polyethylene glycol were found to produce somatic embryos. Calli incubated for 6 h in specified hormone free nutrient medium supplemented with 4% polyethylene glycol was optimal for induction of high frequency SE. Subsequent to stress incubation, the cultures formed only early stage somatic embryos. Supplementation of proline was found essential for the maturation of somatic embryos. Cotyledonary stage somatic embryos were converted into plantlets and subsequently established in garden soil. Semi-quantitative Reverse Transcription-PCR based transcript level analysis of SERK1 gene expression was carried out during different developmental stages of somatic embryogenesis. Expression of SERK1 was specifically associated with the embryogenic calli and calli enriched with globular stage somatic embryos.


英文关键词Embryogenic competence Green gram Mung bean Proline Polyethylene glycol
类型Article
语种英语
国家India ; Czech Republic
收录类别SCI-E
WOS记录号WOS:000427449400005
WOS关键词PLANT-REGENERATION ; ARABIDOPSIS ; LEGUMES ; LIFE
WOS类目Biology
WOS研究方向Life Sciences & Biomedicine - Other Topics
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/210018
作者单位1.Madurai Kamaraj Univ, Sch Biol Sci, Dept Plant Morphol & Algol, Madurai 625021, Tamil Nadu, India;
2.Thiagarajar Coll, Dept Bot, Madurai 625009, Tamil Nadu, India;
3.Masaryk Univ, Fac Sci, Dept Expt Biol, Brno 62500, Czech Republic
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GB/T 7714
Sindhujaa, Vajravel,Gnanaraj, Muniraj,Viji, Maluventhen,et al. Induction of high frequency somatic embryogenesis and analysis of developmental stagewise expression of SERK1 gene during somatic embryogenesis in cultures of Vigna radiata (L.) R.Wilczek[J],2018,56(3):180-193.
APA Sindhujaa, Vajravel,Gnanaraj, Muniraj,Viji, Maluventhen,Karuppanapandian, Thirupathi,&Manoharan, Kumariah.(2018).Induction of high frequency somatic embryogenesis and analysis of developmental stagewise expression of SERK1 gene during somatic embryogenesis in cultures of Vigna radiata (L.) R.Wilczek.INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY,56(3),180-193.
MLA Sindhujaa, Vajravel,et al."Induction of high frequency somatic embryogenesis and analysis of developmental stagewise expression of SERK1 gene during somatic embryogenesis in cultures of Vigna radiata (L.) R.Wilczek".INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY 56.3(2018):180-193.
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