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Murraya koenigii is an important medicinal and aromatic plant. This plant is a native species of arid and semi-arid area of Rajasthan, India. Its leaves used as an analgesic, astringements, anti-dysenteric, antioxidant, hypo-lipidemic and for regulation of fertility. An efficient and reproducible procedure for the large scale propagation of M. koenigii is described. High frequency axillary shoot proliferation was induced in mature nodal explants. Regeneration of shoots (8-10) achieved on Murashige & Skoog (MS) medium (liquid) supplemented with benzylaminopurine (11.09 mu M), kinetin (11.61 mu M) and adenine sulphate (81.44 mu M). Rooting (95%) in regenerated shoots occurred in 3 - 4 weeks of transfer on MS basal medium containing 12.30 mu M indole butyric acid (IBA) and 5.13 mu M indole acetic acid (IAA). In vitro plantlets regenerated from axillary bud proliferation were hardened for four weeks in a green house. The hardened plantlets were transferred to field conditions and high (82 to 85%) survival rate was achieved.