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Human activity on arid lands has been related to oases evolution. The ancient Loulan, an important transportation hub of the ancient Silk Road, developed on an ancient oasis on the west bank of the lake Lop Nur in Xinjiang, China. Previous studies and historical documents suggest that the region has experienced dramatic natural environmental and human activity-related changes over time, transitioning from a particularly prosperous oasis to a depopulated zone with harsh environment after about 1500 a BP (before present, where present = AD 1950). Based on systematic radiocarbon (C-14) dating for natural plant remains and archeological sites in the Loulan area, it was revealed that the region re-experienced oasis environment from 1260 to 1450 cal. AD, corresponding to the Yuan-Ming Dynasties, which is the climate transition stage from the Medieval Warm Period’ to the Little Ice Age’, encompassing a series of pulse-like flood events which cannot be identified from lacustrine deposition due to the limits of sampling resolution and dating. It was found that humans re-occupied the Loulan area and built canals to irrigate farmlands during the period. The more habitable hydrological conditions that resulted from these environmental changes present one major reason for the re-emergence of human activities in the Loulan area.