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Groundwater is a major source of water for domestic, industrial and agricultural uses in many parts of the world, where arid environments are common. Groundwater was collected from 120 wells in a semi-arid and calcareous area, area of Gaziantep, Southeastern Anatolia to determine hydrochemical characteristics, mechanisms for nitrate transport, the concentration of major ions. Relationships between ion concentrations (as meq/L) were analyzed through linear modeling and calculation of coefficients of determination (R-2). The relationships between NO3 and alkaline earth metal ions (M2+) (excess [Ca2+Mg2+] over [HCO3-]) were investigated. There is a strong linear relationship (R-2>0.90) between molar concentrations of the excess M2+ and NO3- ions. Nitrogen compounds originating primarily from microbially-driven processes such as nitrification are expected to react with carbonate minerals (e.g., CaCO3) and may migrate to groundwater as in the following reaction; 2NO(3)((aq))(-) + CaCO3(s) -> Ca(NO3)(2(aq)) + CO3(aq)2-