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Stable isotope-based paleoaltimetry is the most widely used approach for paleoelevation reconstruction. Interpretations of stable isotope data in continental interiors, however, are undermined by surface water isotope compositions that are influenced by multiple factors. Here we present a stable isotope dataset of modern river water samples collected over two summers and one spring from the central Rocky Mountains (Rockies) and the adjacent Great Plains. By examining the spatial and temporal variations of river water delta O-18, delta D and d-excess values, and their relationships with climatic and geographic parameters, as well as through back trajectory analysis of moisture sources, we elucidate the influences of elevation and climatic parameters on the spatiotemporal variation of river water isotopic values. In the Bighorn River drainage, a typical intermontane drainage in the central Rockies, the isotopic difference between highland and lowland rivers is small, which we attribute to highland precipitation that dominates lowland river discharge. In the North Platte River drainage across the central Rockies and Great Plains, the river water delta O-18 values show poor correlation with elevation west of 105 degrees W (central Rockies), but increase as elevation decrease east of 105 degrees W (in the western Great Plains). This eastward increase across the western Great Plains leads to an average oxygen isotope lapse rate of -2.3 parts per thousand/km, which we interpret as being caused primarily by condensation temperature-controlled isotopic fractionation at various elevations, and secondarily by evaporation in the upper reaches of streams that contribute to the North Platte River plus direct contribution of moisture from the Gulf of Mexico in the Great Plains. In this continental interior setting, multiple moisture sources, including recycled continental moisture, contribute to surface water, and evaporation influences river water isotope values to various degrees depending on the relative humidity within an individual river catchment. These results suggest that paleoclimate and atmospheric circulation pattern must be carefully evaluated when applying stable isotope-based paleoaltimetry in continental interiors. Our findings have implications for paleoelevation reconstruction in the study area, including that 1) within the central Rockies, the isotopic difference of river water and unevaporated basinal precipitation can be used to infer paleorelief of the Laramide ranges with respect to the basin floors; 2) along a regional transect crossing the central Rockies and Great Plains, the modern isotope lapse rate of the North Platte River drainage can be used to constrain the paleorelief between the two regions in semi-arid climate. (C) 2018 Elsevier B.V. All rights reserved.