Arid
DOI10.1186/s12917-018-1701-z
Epidemiological survey of sheep as potential hosts for Leishmania in China
Han, Shuai1; Wu, Wei-Ping1; Chen, Kai1; Osman, Israyil2; Kiyim, Kaisar3; Zhao, Jun2; Hou, Yan-Yan2; Wang, Ying1; Wang, Li-Ying1; Zheng, Can-Jun4
通讯作者Wu, Wei-Ping
来源期刊BMC VETERINARY RESEARCH
ISSN1746-6148
出版年2018
卷号14
英文摘要

BackgroundLeishmania parasites cause visceral leishmaniasis (VL), an important infectious disease that is endemic to large parts of the world and often leads to epidemics. Sand flies are the primary transmission vector for the parasite in endemic regions. We hypothesized that sheep might serve as an overlooked reservoir for Leishmania transmission to humans due to the asymptomatic nature of infection in many species. As a preliminary test of this hypothesis, the aim of the present study was to investigate sheep in an area of China that is endemic for the desert sub-type of zoonotic VL and establish if they are potential carriers of Leishmania.ResultsSheep tissue samples were collected from abattoirs in VL endemic areas of Jiashi County, China during the non-transmission season. rK39 immunochromatographic tests were performed to detect the presence of the parasite in blood samples. In addition, DNA was extracted from the blood, and used for detection of the Leishmania-specific internal transcribed spacer-1 (ITS-1) genomic region using a nested polymerase chain reaction (PCR) approach. PCR products were further analyzed to identify restriction fragment-length polymorphism patterns and representative sequences of each pattern were selected for phylogenetic analysis. The rK-39 and nested PCR data indicated positive detection rates for Leishmania in sheep of 26.32 and 54.39%, respectively. The phylogenetic analysis revealed that all of the samples belonged to the species L. infantum and were closely related to strains isolated from human infections in the same area.ConclusionsSheep could be a potential host for Leishmania in VL endemic areas in China and may be an overlooked reservoir of human VL transmission in this region. To further confirm livestock as a potential host, further verification is required using a sand fly biting experiment.


英文关键词Leishmania Visceral leishmaniasis China Phylogenetics Sheep Reservoir
类型Article
语种英语
国家Peoples R China
收录类别SCI-E
WOS记录号WOS:000452006400003
WOS关键词UYGUR AUTONOMOUS REGION ; VISCERAL LEISHMANIASIS ; CUTANEOUS-LEISHMANIASIS ; INFANTUM INFECTION ; PCR DIAGNOSIS ; KALA-AZAR ; BIHAR ; DOGS ; AREA
WOS类目Veterinary Sciences
WOS研究方向Veterinary Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/208198
作者单位1.Minist Sci & Technol, Natl Ctr Int Res Trop Dis,Minist Hlth, Natl Inst Parasit Dis,Chinese Ctr Dis Control & P, WHO Collaborating Ctr Trop Dis,Key Lab Parasite &, Shanghai 200025, Peoples R China;
2.Xinjiang Uygur Autonomous Reg Ctr Dis Control & P, Urumqi, Peoples R China;
3.Kashgar Prefectural Ctr Dis Control & Prevent, Kashgar, Peoples R China;
4.Chinese Ctr Dis Control & Prevent, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Han, Shuai,Wu, Wei-Ping,Chen, Kai,et al. Epidemiological survey of sheep as potential hosts for Leishmania in China[J],2018,14.
APA Han, Shuai.,Wu, Wei-Ping.,Chen, Kai.,Osman, Israyil.,Kiyim, Kaisar.,...&Zheng, Can-Jun.(2018).Epidemiological survey of sheep as potential hosts for Leishmania in China.BMC VETERINARY RESEARCH,14.
MLA Han, Shuai,et al."Epidemiological survey of sheep as potential hosts for Leishmania in China".BMC VETERINARY RESEARCH 14(2018).
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