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Lithological discrimination of Neoproterozoic rocks occupying Nugrus-Hafafit area, South Eastern Desert of Egypt, has been carried out using Operational Land Imager (OLI) and Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) sensors’ imagery data. The applicable processing enhancement techniques include optimum index factor (OIF), band ratioing, principal component analysis (PCA), and minimum noise fraction (MNF) transform. The area comprises varieties of low-grade metamorphosed ophiolitic melange and island-arc rocks, thrusting over high-grade metamorphic gneissic core complexes, and intruded by syn-, late-, and post-tectonic granitoids. The OLI band ratio 6/7 discriminates clearly the ophiolitic serpentinites-talc-carbonate rocks, while 4/5 ratio image is able to separate between mafic and felsic rocks. Moreover, the ASTER band ratio 6/8 is used to distinguish the amphibole-bearing rocks, including amphibolite and hornblende gneiss. The OLI and ASTER second principal component (PC2) images reflect the contrast spectral behavior of ophiolitic melange rocks through visible-near-infrared (VNIR) and shortwave (SWIR) regions. The OLI-PC3 shows the ability to delineate the Fe-rich rocks, including amphibolite and metamafics, while ASTER-PC3 is effective for quartz-feldspathic granites and psammitic gneisses. Visual interpretation and integration of processed data with petrography and field investigation resulted in complete differentiation for the different lithologies and creation of a new detailed geological map of Nugrus-Hafafit area.