Arid
DOI10.1039/c8an00939b
A bio-inspired photonic nitrocellulose array for ultrasensitive assays of single nucleic acids
Chi, Junjie; Ma, Biao; Dong, Xing; Gao, Bingbing; Elbaz, Abdelrahman; Liu, Hong; Gu, Zhongze
通讯作者Liu, Hong ; Gu, Zhongze
来源期刊ANALYST
ISSN0003-2654
EISSN1364-5528
出版年2018
卷号143期号:19
英文摘要

Here we report a bio-inspired photonic nitrocellulose array for ultrasensitive nucleic-acid detection. The patterned photonic nitrocellulose array is inspired by the Stenocara beetle living in the desert, which can collect water on its bumpy back surface from early morning fogs so that spontaneous generation of separated reaction droplets for loop-mediated isothermal amplification (LAMP)-based detection is enabled. Owing to the slow-photon effect of the photonic nitrocellulose, the fluorescence signal of calcein produced during the LAMP reaction can be effectively enhanced (up to 32 fold), which results in dramatically improved sensitivity for the detection of single nucleic acids in 40 min. We demonstrate that Staphylococcus aureus (SA) DNA can be quantitatively detected with a limit-of-detection of 0.60 copy per L. The consumption of reagents and sample is also remarkably reduced owing to the highly decreased dead volume of the nitrocellulose substrate. Therefore, this bio-inspired photonic nitrocellulose array is promising for carrying out inexpensive, ultrasensitive, and high-throughput nucleic-acid detection under resource-limited settings.


类型Article
语种英语
国家Peoples R China
收录类别SCI-E
WOS记录号WOS:000448337600004
WOS关键词MEDIATED ISOTHERMAL AMPLIFICATION ; DNA AMPLIFICATION ; EXTRACTION ; CRYSTAL ; QUANTIFICATION ; MICROCAPILLARY ; ENHANCEMENT ; DIAGNOSTICS ; BIOLOGY ; DROP
WOS类目Chemistry, Analytical
WOS研究方向Chemistry
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/207522
作者单位Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714
Chi, Junjie,Ma, Biao,Dong, Xing,et al. A bio-inspired photonic nitrocellulose array for ultrasensitive assays of single nucleic acids[J],2018,143(19).
APA Chi, Junjie.,Ma, Biao.,Dong, Xing.,Gao, Bingbing.,Elbaz, Abdelrahman.,...&Gu, Zhongze.(2018).A bio-inspired photonic nitrocellulose array for ultrasensitive assays of single nucleic acids.ANALYST,143(19).
MLA Chi, Junjie,et al."A bio-inspired photonic nitrocellulose array for ultrasensitive assays of single nucleic acids".ANALYST 143.19(2018).
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