Arid
DOI10.1007/s13205-017-1075-0
Phylogeny, novel bacterial lineage and enzymatic potential of haloalkaliphilic bacteria from the saline coastal desert of Little Rann of Kutch, Gujarat, India
Bhatt, Hitarth B.; Gohel, Sangeeta D.; Singh, Satya P.
通讯作者Singh, Satya P.
来源期刊3 BIOTECH
ISSN2190-572X
EISSN2190-5738
出版年2018
卷号8
英文摘要

This report describes cultivation-dependent diversity, phylogeny and enzymatic potential of the haloalkaliphilic bacteria isolated from the unvegetated desert soil of yet unexplored, saline desert of Little Rann of Kutch (LRK), India. The LRK is a unique ecosystem displaying a combination of Dry Rann and Wet Rann. A total of 25 bacteria were isolated and characterized on the basis of colony morphology, biochemical profile, sugar utilization, secretion of the extracellular enzymes and antibiotic sensitivity. Further, the identification and phylogenetic relatedness of 23 bacteria were established by the analysis of 16S rRNA gene sequences. The phylogenetic analysis indicated that the isolates belong to the phylum Firmicutes, comprising low G + C, Gram-positive bacteria, with different genera: Bacillus (similar to 39%), Staphylococcus (similar to 30%), Halobacillus (similar to 13%), Virgibacillus (similar to 13%), Oceanobacillus (similar to 4%). Majority of the bacterial isolates produced proteases (30% isolates) followed by cellulases (24% isolates), CMCases (24% isolates) and amylases (20% isolates). Halobacillus, Virgibacillus and Bacillus predominantly produced hydrolases, while many produced multiple enzymes at high salinity and alkaline pH. Highest antibiotic resistance was observed against Ampicillin and Penicillin (32%) followed by Cefaclor (20%); Colistin, Cefoperazone and Cefotaxime (16%); Cefuroxime (12%); Gentamycin and Cefixime (8%); Erythromycin, Cefadroxil, Azithromycin, Cotrimoxazole, Amoxycillin, Norfloxacin, Cefpodoxime, Amikacin and Augmentin (4%). KJ1-10-99 and KJ1-10-93 representing < 97% of 16S rRNA gene sequence similarity belong to a novel lineage within the family Bacillaceae. Comparison of the phenogram and phylogram revealed the contradiction of the phenogram pattern and the phylogenetic placement of the isolates. The isolates belonging to same species have shown considerable phenotypic variation. The study on the cultivable haloalkaliphilic bacteria of an unexplored enigmatic niche reflects ecological and biotechnological significance.


英文关键词Haloalkaliphilic bacteria Extracellular enzymes Phenogram Coastal saline desert Bacterial diversity 16S rRNA gene sequencing Little Rann of Kutch Novel lineage
类型Article
语种英语
国家India
收录类别SCI-E
WOS记录号WOS:000419534800003
WOS关键词GREAT SALT PLAINS ; MICROBIAL DIVERSITY ; SP NOV. ; ALKALINE PROTEASE ; GENETIC DIVERSITY ; ATACAMA DESERT ; SOIL ; HALOTOLERANT ; BIODIVERSITY ; SEQUENCES
WOS类目Biotechnology & Applied Microbiology
WOS研究方向Biotechnology & Applied Microbiology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/207140
作者单位Saurashtra Univ, CAS Dept Biosci, UGC, Rajkot 360005, Gujarat, India
推荐引用方式
GB/T 7714
Bhatt, Hitarth B.,Gohel, Sangeeta D.,Singh, Satya P.. Phylogeny, novel bacterial lineage and enzymatic potential of haloalkaliphilic bacteria from the saline coastal desert of Little Rann of Kutch, Gujarat, India[J],2018,8.
APA Bhatt, Hitarth B.,Gohel, Sangeeta D.,&Singh, Satya P..(2018).Phylogeny, novel bacterial lineage and enzymatic potential of haloalkaliphilic bacteria from the saline coastal desert of Little Rann of Kutch, Gujarat, India.3 BIOTECH,8.
MLA Bhatt, Hitarth B.,et al."Phylogeny, novel bacterial lineage and enzymatic potential of haloalkaliphilic bacteria from the saline coastal desert of Little Rann of Kutch, Gujarat, India".3 BIOTECH 8(2018).
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