干旱区生态环境知识资源中心(Arid): 人端粒酶逆转录酶在子宫颈癌组织中的表达变化及其意义

Arid

	人端粒酶逆转录酶在子宫颈癌组织中的表达变化及其意义
其他题名	Expression of human telomerase reverse transcriptase in cervix cancer and its significance
	奚玲; 朱涛; 吴鹏; 徐茜; 黄磊; 李科珍; 卢运萍; 马丁
来源期刊	中华妇产科杂志
ISSN	0529-567X
出版年	2005
卷号	40 期号:6 页码:407-410
中文摘要	目的研究端粒酶在宫颈癌及其癌前病变组织中的表达,探讨人端粒酶逆转录酶(hTERT)作为宫颈癌早期诊断标志物的可能性.方法采用RT-PCR方法测定端粒酶mRNA在3株宫颈癌细胞、2株正常的人二倍体细胞、38例宫颈癌、16例宫颈上皮内瘤变(CIN)和20例正常宫颈组织中的表达水平;端粒重复序列扩增-酶联免疫吸附法测定端粒酶活性;免疫组化链酶菌抗生物素蛋白-过氧化物酶(SP)连接法检测3株宫颈癌细胞、2株正常的人二倍体细胞、21例正常宫颈组织、25例CIN和55例宫颈癌切片中hTERT蛋白的表达水平.结果 hTERT mRNA在3株宫颈癌细胞中均表达,而在2株正常的人二倍体细胞中无表达,宫颈癌组织中的阳性表达率为81.6%,高于CIN的37.5%和正常宫颈组织的5.0%,差异有统计学意义(P<0.05);hTERT mRNA的阳性表达率与端粒酶活性有相关性(P<0.01);hTERT蛋白在3株宫颈癌细胞中有表达,2株正常的人二倍体细胞中无表达,正常宫颈组织中阳性表达率为4.8%、CIN为28.0%,均低于宫颈癌组织的65.5%,差异有统计学意义(P<0.05).结论宫颈癌组织和细胞中hTERT mRNA和蛋白阳性表达率均高于正常宫颈组织,其有可能作为宫颈癌早期诊断的标志物.
英文摘要	Objective To investigate the expression of human telomerase reverse transcriptase (hTERT) mRNA and protein in cervix cancer, cervical intraepithelial neoplasia (CIN) and normal cervix. Methods Expression of hTERT mRNA and the other two suhunits of telomerase, human telomerase RNA component (hTR) , human telomerase-associated protein (hTPl) was determined by RT-PCR in 3 cervix cancer cell lines, 2 diploid cell lines, 38 cases of cervix cancer, 16 cases of CIN and 20 cases of normal cervix. Telomerase activity was also examined by telomeric repeat amplification protocol enzyme-linked immunosorbent assay (TRAP-ELISA). Expression of hTERT protein was detected in all the cell lines and 101 oases of paraffinized cervix tissue sections. Results hTERT mRNA expression was detected in all of the three cervix cancer cell lines, 81. 6% of cervix cancer, 37. 5% of CIN, 5. 0% of normal cervix, while in neither of the two diploid cell lines. The other two subunits of telomerase were prevalently expressed in all of the cell lines and most cervix tissues. There was a strong correlation between hTERT mRNA expression and telomerase activity. Immunostaining also revealed that hTERT protein was expressed in all three cervix cancer cell lines, 65. 5% of cervix cancer, 28. 0% of CIN and 4. 8% of normal cervix. Conclusion Up-regulation of hTERT may play an important role in the development of CIN and cervix cancer, hTERT could be used as an early diagnostic biomarker for cervix cancer.
中文关键词	端粒,末端转移酶 ; 宫颈肿瘤
英文关键词	Telomerase Cervix neoplasms
语种	中文
国家	中国
收录类别	CSCD
WOS类目	OBSTETRICS GYNECOLOGY
WOS研究方向	Obstetrics & Gynecology
CSCD记录号	CSCD:2118716
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/207070
作者单位	华中科技大学同济医学院附属同济医院妇产科, 武汉, 湖北 430030, 中国
推荐引用方式 GB/T 7714	奚玲,朱涛,吴鹏,等. 人端粒酶逆转录酶在子宫颈癌组织中的表达变化及其意义[J],2005,40(6):407-410.
APA	奚玲.,朱涛.,吴鹏.,徐茜.,黄磊.,...&马丁.(2005).人端粒酶逆转录酶在子宫颈癌组织中的表达变化及其意义.中华妇产科杂志,40(6),407-410.
MLA	奚玲,et al."人端粒酶逆转录酶在子宫颈癌组织中的表达变化及其意义".中华妇产科杂志 40.6(2005):407-410.