干旱区生态环境知识资源中心(Arid): 全血中苯骈二氮杂类药物的胶束电动色谱法分析

Arid

	全血中苯骈二氮杂类药物的胶束电动色谱法分析
其他题名	Determination of benzodiazepines in human whole blood by micellar electrokinetic capillary chromatography
	张玉荣 1; 金琦芸 2; 梁晨 3; 郭幼梅 2; 曾立波 3
来源期刊	中国法医学杂志
ISSN	1001-5728
出版年	2005
卷号	20 期号:2 页码:80-82
中文摘要	目的建立用固相萃取胶束电动毛细管色谱法测定人体全血中苯骈二氮杂类药物的方法.方法全血以Oasis小柱提取,以克仑特罗为内标,采用未涂层毛细管(75mum ID50.2cm,有效分离长度为40cm),缓冲液为30mmol/L SDS15mmol/L硼砂15mmol/L磷酸盐(pH8.2)18%甲醇.进样条件:压力进样0.5psi1os,分离电压为25kV,柱温25℃,检测波长为230nm.结果本法分离效率高,9种苯骈二氮杂类药物的最低检测浓度为5～50ng/ml;血药浓度的线性范围为0.02～1.6mug/ml,日内、日间精密度＜12%.结论本法简便、高效、可靠.
英文摘要	Objective To develop a method for simultaneous determination of benzodiazepines in hu- ;man whole blood by SPE-Micellar electrokinetic capillary chromatography. Methods With the Clenbuterol as \|internal standard, Oasis column was used to extract the drugs from whole blood. The separation was per-formed on a fused-silica capillary of 75\|xm ID x50. 2cm(40cm of effect length). The running buffers weresequentially used as 15mmol/L phosphates->-15mmo]/L sodium borate (pH8. 2) -?-30 mmol/L SDS,and18% methanol served as an organic modifier. Sample solution was injected with pressure mode, and the run-ning voltage was 25kV. The detection wavelength was set at 230nm. Results The linear ranges of the calibra-tion curves were from 0. 02 to 1. 6\|xg/ml,and the limits of detection ranged between 5 ng/ml and 50 ng/ml. IThe within-day and between-day precision was less than 12%. Conclusion The method developed for deter- imination of benzodiazepines in human whole blood is effective, simple and reliable, with which 9 benzodiaz- \|epines may be simultaneously separated.
中文关键词	法医毒物分析 ; 固相萃取 ; 胶束电动色谱法 ; 苯骈二氮杂类药 ; 全血
英文关键词	Forensic toxicology analysis SPE Micellar electrokinetic capillary chromatography Benzodiazepines Whole blood
语种	中文
国家	中国
收录类别	CSCD
WOS类目	LAW
WOS研究方向	Government & Law
CSCD记录号	CSCD:1918716
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/206915
作者单位	1.上海市公安局刑事科学技术研究所, 上海 200083, 中国; 2.复旦大学药学院药物分析教研室, 上海 200032, 中国; 3.上海市刑事科学技术研究所, 上海 200083, 中国
推荐引用方式 GB/T 7714	张玉荣,金琦芸,梁晨,等. 全血中苯骈二氮杂类药物的胶束电动色谱法分析[J],2005,20(2):80-82.
APA	张玉荣,金琦芸,梁晨,郭幼梅,&曾立波.(2005).全血中苯骈二氮杂类药物的胶束电动色谱法分析.中国法医学杂志,20(2),80-82.
MLA	张玉荣,et al."全血中苯骈二氮杂类药物的胶束电动色谱法分析".中国法医学杂志 20.2(2005):80-82.