干旱区生态环境知识资源中心(Arid): 濒危植物矮沙冬青的等位酶多样性及居群分化

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	濒危植物矮沙冬青的等位酶多样性及居群分化
其他题名	Allozyme Diversity and Population Differentiation in an Endangered Plant, Ammopiptanthus nanus (Leguminosae)
	陈国庆 1; 黄宏文 2; 葛学军 1
来源期刊	武汉植物学研究
ISSN	1000-470X
出版年	2005
卷号	23 期号:2 页码:131-137
中文摘要	采用超薄平板微型聚丙烯酰胺等电聚焦技术，对珍稀濒危植物矮沙冬青(Ammopiptanthus nanus)6个自然居群的等位酶遗传多样性进行了初步研究。8个酶系统、l9个酶位点的检测结果表明，矮沙冬青具有较低的遗传变异水平。物种水平上的遗传多样性参数为：P=21．05％，A=1．21，Ht=0．040；居群水平上的平均遗传多样性为：P=14．04％，A=1．14(0．04)，Ht=0．036(0．018)，比长寿命多年生木本的狭域分布种和特有种的多样性水平都低。F-统计量分析显示，矮沙冬青在居群水平(F15=0．129)和总居群水平(FIT=0．202)上都表现为杂合子缺乏；Fsr=0．085表明居群问遗传分化较小，这与居群间较高的遗传一致度(I=0．997)的结果一致。据研究结果推测矮沙冬青进化历史上经历过遗传瓶颈，是造成遗传多样性水平偏低的重要原因。基于研究结果，提出应当重点保护遗传变异相对丰富的何依儿佣克尔(KE)居群，并且可将其作为引种的种源地。
英文摘要	Ammopiptanthus nanus is a rare and endangered species, native to desert habitats in Northern China. Allozyme diversity and population differentiation were evaluated for six natural populations using isoelectric focusing in thin-layer polyacrylamide slab gels. A total of 19 loci from eight enzyme systems were detected. An extreme low level of genetic variation was revealed both at the species levelCA = 1. 21,P = 21. 05% ,He = 0. 040)and at population level(P = 14. 04% , A = 1.14(0. 04), He - 0.036 (0.018) ), which were lower than those of long-lived perennial woody and narrowly distributed species. Wright’s F-statistics analysis indicated a deficit of het-erozygote both at population level(F7s = 0. 129) and at the total population level (F;T=0. 202). A low level of genetic differentiation among populations detected (FST=0. 085) was consistent with a high level of genetic identity among the populations (/= 0. 997). The history of bottleneck effect was speculated to be the main course for the current genetic structure in A. nanus. Consequently, conservation concerns of this species were discussed and conservation recommendations are suggested based on the results obtained from the study.
中文关键词	濒危物种 ; 矮沙冬青 ; 等位酶 ; 遗传多样性 ; 居群分化 ; 遗传瓶颈
英文关键词	Endangered species Ammopiptanthus nanus Allozyme Genetic diversity Population differentiation Genetic bottleneck
语种	中文
国家	中国
收录类别	CSCD
WOS类目	BIODIVERSITY CONSERVATION
WOS研究方向	Biodiversity & Conservation
CSCD记录号	CSCD:2234194
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/206739
作者单位	1.中国科学院华南植物园, 广州, 广东 510650, 中国; 2.中国科学院武汉植物园, 武汉, 湖北 430074, 中国
推荐引用方式 GB/T 7714	陈国庆,黄宏文,葛学军. 濒危植物矮沙冬青的等位酶多样性及居群分化[J],2005,23(2):131-137.
APA	陈国庆,黄宏文,&葛学军.(2005).濒危植物矮沙冬青的等位酶多样性及居群分化.武汉植物学研究,23(2),131-137.
MLA	陈国庆,et al."濒危植物矮沙冬青的等位酶多样性及居群分化".武汉植物学研究 23.2(2005):131-137.