干旱区生态环境知识资源中心(Arid): 藜对干旱胁迫的生理生化反应

Arid

	藜对干旱胁迫的生理生化反应
其他题名	Physiological and biochemical responses of Chenopodium album to Drought Stresses
	孙存华 1; 李扬 1; 贺鸿雁 1; 孙东旭 2; 杜伟 1; 郑曦 1
来源期刊	生态学报
ISSN	1000-0933
出版年	2005
卷号	25 期号:10 页码:2556-2561
中文摘要	干旱是植物最易遭受的胁迫之一，每年由于干旱胁迫给农业造成损失几乎相当于其他所有环境因子胁迫所造成的损失的总和。通过人工控制水分模拟干旱来研究生长期的藜对干旱胁迫的生理生化反应，以期望为干旱农业的高效生产提供理论依据。以盆栽的藜为材料，用控制浇水的方法分对照、轻度胁迫、中度胁迫、重度胁迫4个组，研究了不同程度干旱胁迫对藜叶片的水分状况、渗透调节物质、活性氧代谢以及内生保护系统的影响。结果表明：在干旱胁迫下，藜叶片相对含水量（RWC）、自由水含量（FWC）下降，束缚水含量（BWC）升；可溶性糖、脯氨酸、K^＋、Ca^2＋含量增加，表现出藜对适度干旱有一定的适应性。但重度干旱胁迫，O2^.-产生速率和丙二醛（MDA）含量显著提高，导致膜损伤，质膜透性上升；超氧化物歧化酶（SOD）、过氧化物酶（POD）活性先上升，后下降；抗坏血酸（ASA）含量降低。过分干旱胁迫对藜会造成一定伤害。
英文摘要	Drought is one of the stress conditions that often severely effects plant growth. The loss of agricultural production due to drought stress is estimated to nearly equal the total losses caused by all other environmental stresses. In this study, the physiological and biochemical responses of Chenopodium album L. under artificially simulated drought stresses were examined to provide a theoretical basis for effectively increasing agricultural production in arid areas. C. album L. was grown from freshly harvested seeds under natural light. Twelve pots of plants showing similar growth were divided into 4 groups and treated with different levels of drought stress by controlled irrigation： no stress （control）, watered once a week for 4 weeks; light stress, watered once a week for the first 3 weeks; moderate stress, watered once a week for the first 2 weeks; severe stress, watered once in the first week only. Each time the water was allowed to fully saturate the moisture capacity of soil. The pots were protected from exposure to rain. Leaf samples were taken after the fourth week （the final week） of the experiment. The following parameters were determined： relative water content （RWC）, free water content（FWC）, bound water content（BWC）, soluble sugars, free proline, K^＋, Ca^2＋, malondialdehyde （MDA） and free ascorbic acid （ASA）,membrane permeability, the production rate of O2^.-, and activities of superoxide dismutase （SOD） and peroxidase （POD）. The relative water content in leaves of the control, lightly-stressed, moderately-stressed and severelystressed plants were 94.07%, 87.01%, 76.35% and 64.03%, respectively. The water content in soil 5% 7 cm under the surface in the 4 groups of pots at the same time were 23.33%, 16.92%, 10.82% and 7.73%, respectively. Under moderate drought stress, the RWC and FWC in leaves were decreased while the BWC was increased. The activities of SOD and POD in leaves reached the highest level among the four treatments. Membrane permeability, MDA content and the O2^.- production rate in leaves declined; whereas osmoregulatory molecules such as soluble sugars, proline, K^＋ and Ca^2＋ in leaves accumulated rapidly, indicating that C. album has the ability to adapt to drought stress by regulating the internal osmolarity and protecting the membrane. Under severe drought stress, however, the O2^.- production rate and the MDA content increased remarkably, causing membrane damage and increasing membrane permeability of leaf cells. The activities of SOD and POD were initially increased as compared to those under the moderate drought stress and then declined; ASA content was also decreased. These results suggest that C. album is vulnerable to damage by severe drought stress.
中文关键词	藜 ; 干旱胁迫 ; 渗透调节物质 ; 膜脂质过氧化 ; 保护酶
英文关键词	Chenopodiurn album drought stress osmoregulatory molecules membrane lipid peroxidation protective enzyme
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ECOLOGY
WOS研究方向	Environmental Sciences & Ecology
CSCD记录号	CSCD:2206340
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/206554
作者单位	1.徐州师范大学生命科学学院, 徐州, 江苏 221116, 中国; 2.江苏省药用植物生物技术重点实验室, 江苏省药用植物生物技术重点实验室, 徐州, 江苏 221116, 中国
推荐引用方式 GB/T 7714	孙存华,李扬,贺鸿雁,等. 藜对干旱胁迫的生理生化反应[J],2005,25(10):2556-2561.
APA	孙存华,李扬,贺鸿雁,孙东旭,杜伟,&郑曦.(2005).藜对干旱胁迫的生理生化反应.生态学报,25(10),2556-2561.
MLA	孙存华,et al."藜对干旱胁迫的生理生化反应".生态学报 25.10(2005):2556-2561.