干旱区生态环境知识资源中心(Arid): 液相色谱-串联四级杆质谱对食品中丙烯酰胺的测定研究

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	液相色谱-串联四级杆质谱对食品中丙烯酰胺的测定研究
其他题名	Determination of Acrylamide in Foods by Liquid Chromatography/Tandem Quadrupole Mass Spectrometry
	樊祥 1; 方晓明 2; 陈家华 2; 吕敬慈 1; 张星漪 3
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2005
卷号	24 期号:3 页码:82-85
中文摘要	用液相色谱-串联四级杆质谱法检测食品中丙烯酰胺的含量.添加氘标记的内标d3-丙烯酰胺于样品中,经纯水提取,用Oasis HLB和Bond Elut Accucat固相萃取柱净化.Zorbax XDB-C18色谱柱分离,流动相为MeOH-H2O(体积比1:99)含0.1%HAc溶液,流速0.19 mL/min.电喷雾正离子MRM模式检测:丙烯酰胺m/z 7255,内标m/z 7558.内标法定量,方法定量下限(LOQ,S/N＞10)为50 ngg/,在质量浓度0.005～100 mug/mL范围内,峰面积与浓度成良好线性(r＞0.998).本法快速、准确、检出限低、实用性强.
英文摘要	Aetylamide in foods was determined by liquid chromatography with tandem quadrupole mass spec-Irometry, A sample spiked with deuterium-labeled acrylamide-cb (internal standard) was extracted with wa-ter, and centrifugated at high speed. An aliquot of the supernatant was filtered through a Maxi-Spin(0.45mum PVDF) filter. The filtrate was purified by SPE with Oasis HLB cartridge and Bond Elut Accucat cartridge. Chromatographic separation was carried out on a Zorbax XDB-Cis column(2.1 * 150 mm, 5 p.m) with elution of methanol - water(1 : 99 by volume) containing 0.1% acetic acid at a flow rate of 0.19 mL/min. The mass spectrometer was operated in the positive electrospray ionization mode. Quantition was performed using multiple reaction monitoring of the product ion transitions m/z 7255 for aciylamide and rn/z 7558 for aciylamide-d3. Linearity of peak area was obtained over a wide range of concentrations (from 0.005 to 100mug/mL), and the correlation coefficient was greater than 0.998. The method had a limit of quantisation (LOQ, S/N > 10) of 50 ng/g. The method was sensitive, reliable, selective and reproducible.
中文关键词	液相色谱 ; 串联四级杆质谱 ; 丙烯酰胺 ; 食品
英文关键词	Liquid chromatography Tandem quadrupole mass spectrometry Acrylamide Food
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:1966362
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/206071
作者单位	1.上海大学,化学系, 上海 200436, 中国; 2.上海出入境检验检疫局, 上海 200135, 中国; 3.上海水产大学,食品学院, 上海 200090, 中国
推荐引用方式 GB/T 7714	樊祥,方晓明,陈家华,等. 液相色谱-串联四级杆质谱对食品中丙烯酰胺的测定研究[J],2005,24(3):82-85.
APA	樊祥,方晓明,陈家华,吕敬慈,&张星漪.(2005).液相色谱-串联四级杆质谱对食品中丙烯酰胺的测定研究.分析测试学报,24(3),82-85.
MLA	樊祥,et al."液相色谱-串联四级杆质谱对食品中丙烯酰胺的测定研究".分析测试学报 24.3(2005):82-85.