干旱区生态环境知识资源中心(Arid): 系统性血管炎抗内皮细胞抗体的检测及其与病情活动的相关性

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	系统性血管炎抗内皮细胞抗体的检测及其与病情活动的相关性
其他题名	Anti-endothelial cell antibodies in systemic vasculitis: detection and correlation with disease activity
	曾学军; 朱卫国; 邓学新; 唐福林; 董怡
来源期刊	中华医学杂志
ISSN	0376-2491
出版年	2004
卷号	84 期号:19 页码:1629-1632
中文摘要	目的研究抗内皮细胞抗体(AECA)在系统性血管炎患者血清中的检出率,分析其与病情活动的相关性,比较两者差异有无显著意义,初探AECA的分类.方法采用Cyto-ELISA法,分别以EA.hy926及HMEC-1为底物,检测122例系统性血管炎患者血清中AECA,与临床资料行相关性分析,并比较两种底物细胞所测AECA有无差异.结果 EA.hy926为底物时系统性血管炎(33.61%)及系统性红斑狼疮(SLE)患者(32.35%),AECA阳性率显著高于类风湿关节炎(RA)(2.50%)患者及正常人(4.76%);HMEC-1为底物细胞时系统性血管炎患者(37.70%)显著高于RA患者(7.5%)及正常人(9.52%),SLE患者的AECA阳性率(61.75%)显著高于其他各组.其他组间差异无显著意义.AECA与红细胞沉降率相关,与其他生化指标无相关性.伯明翰血管活动度评分(BVAS)与AECA P/N成正相关.对两种底物细胞所测AECA 值显著相关,符合率92.62%.结论 AECA在系统性血管炎有较高的检出率,可反映病情活动度;AECA在系统性血管炎可否分为针对大小血管内皮细胞两类,有待进一步研究.
英文摘要	Objective To investigate the prevalence of anti-endothelial cell antibodies (AECA) in systemic vasculitis and to assess the correlation between AECA and disease activity, and try to discuss the classification of AECA. Methods Cyto-ELISA with EA. hy926 and HMEC-1, two immortalizea cell lines, as substrates, was applied to detect AECA in 122 cases of systemic vasculitis, including 43 cases of Behcet disease, 19 cases of Takayasu arteritis, 19 cases of Wegener’s granulomatosis, 11 cases of microscopic polyangiitis, 9 cases of polyarteritis nodosa,3 cases of Churg-Strause syndrome,2 cases of giant cell arteritis and other 16 cases of which could not be classified clearly. Patients with SLE, RA, and fever of unkown origin, and normal persons were used as control groups. Then the associations of AECA to laboratory findings and clinical disease activity (scored by BVAS) were analyzed. Furthermore, the AECA value from the two different substrate cells were anyalyzed for correlation and difference. Results With either EA. hy926 or HMEC-1 as substrates, the AECA prevalence rates of systemic vasculitis ( respectively 33.61% and 37. 70% ) were significantly higher than those of the normal and RA groups (less than 10 % ) , and the prevalence of AECA in SLE (61. 75% ) was higher than that of systemic vasculitis (37. 70% ) when using HMEC-1 as substrates. AECA was found to correlate very well with ESR in 122 oases of systemic vasculitis and with BVAS in 40 cases of small systemic vasuculitides, including Wegener’s disease, microscopic polyangiitis, Churg-Strause syndrome and so on. The pair AECA values with EA. hy926 and HMEC-1 as substrate cells were found to be correlated significantly, and the matching rate was 92.62%. Conclusion Prevalence of AECA in systemic vasculitis is high. AECA indicates the clinical disease activity. As to the assumption of classification of AECA into antibodies against microvascular and macrovascular endothelial cells, futher study need to be done to prove or disprove it.
中文关键词	内皮 ; 抗体 ; 血管炎 ; 酶联免疫吸附测定
英文关键词	Endothelium Antibodies Vasculitis Enzyme-linked immunosorbent assay
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:1682701
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/205846
作者单位	中国医学科学院,中国协和医科大学,北京协和医院风湿免疫内科, 100730
推荐引用方式 GB/T 7714	曾学军,朱卫国,邓学新,等. 系统性血管炎抗内皮细胞抗体的检测及其与病情活动的相关性[J],2004,84(19):1629-1632.
APA	曾学军,朱卫国,邓学新,唐福林,&董怡.(2004).系统性血管炎抗内皮细胞抗体的检测及其与病情活动的相关性.中华医学杂志,84(19),1629-1632.
MLA	曾学军,et al."系统性血管炎抗内皮细胞抗体的检测及其与病情活动的相关性".中华医学杂志 84.19(2004):1629-1632.