干旱区生态环境知识资源中心(Arid): 低氧诱导因子2和血管内皮生长因子在膀胱癌中的表达及意义

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	低氧诱导因子2和血管内皮生长因子在膀胱癌中的表达及意义
其他题名	Expression and significance of endothelial Per-ARNT-Sim domain protein-1 ( EPAS-1 )/hypoxia-inducible factor-2alpha(HIF-2alpha) and vascular endothelial growth factor (VEGF) in transitional cell carcinoma of bladder
	陈小勇 1; 段建敏 1; 卢建中 2; 邱中瑾 1; 史庭凯 2
来源期刊	中华泌尿外科杂志
ISSN	1000-6702
出版年	2004
卷号	25 期号:12 页码:844-847
中文摘要	目的探讨低氧诱导因子2(EPAS1/HIF-2alpha)和血管内皮生长因子(VEGF)在膀胱移行细胞癌中的表达意义.方法应用免疫组织化学技术检测60例膀胱移行细胞癌[Ⅰ级28例,Ⅱ级12例,Ⅲ级20例;浅表性膀胱癌(Tis～T1)29例,浸润性(T2～T4)31例]和8例正常膀胱组织中EPAS1/HIF-2alpha和VEGF的表达情况,chi2检验分析其表达与膀胱癌分级和分期间的关系.结果EPAS1/HIF-2alpha和VEGF在正常膀胱组织中不表达,而在膀胱癌组织表达较强.60例膀胱癌标本中EPAS1/HIF-2alpha阳性表达34例,阴性26例.病理分级Ⅰ级标本阳性表达4例(14.3%),Ⅱ级11例(91.7%),Ⅲ级19例(95.0%).浅表性癌中阳性5例(17.2%),浸润性癌中阳性29例(93.5%).EPAS1/HIF-2alpha与肿瘤的病理分级(r=0.862,P《0.001)和临床分期(r=0.805,P《0.001)密切相关.60例膀胱癌标本中VEGF阳性表达44例.病理分级Ⅰ级标本阳性表达12例(42.8%),Ⅱ级12例、Ⅲ级19例均阳性表达.浅表性膀胱癌中阳性表达14例(48.3%),浸润性癌中阳性表达30例(96.8%),VEGF表达与肿瘤病理分级(r=0.841,P《0.001)和临床分期(r=0.819,P《0.001)密切相关.EPAS1/HIF-2alpha表达与VEGF表达密切相关(r=0.537,P《0.001).结论EPAS1/HIF-2alpha表达与膀胱癌的生长、浸润密切相关;对EPAS1/HIF-2的研究开发可能为浸润性膀胱癌提供新的诊断标记物或基因治疗效靶.
英文摘要	Objective To evaluate the expression of endotheliai Per-ARNT-Sim domain protein-1 (EPAS-1)/hypoxia-inducible factor-2a( HIF-2a) and its relationship with vascular endotheliai growth factor (VEGF) in bladder transitional cell carcinoma (BTCC) . Methods The expression of EPAS-l/HIF-2a and VEGF by im-raunohistochemical staining was examined in 60 cases of BTCC (Grade I in 28 cases,Grade I in 12,Grade III in 20) and 8 subjects with normal urothelium. Of the 60 cases 29 had superficial ( ^pT, ) bladder cancer and 31 had invasive ( SpT2 bladder cancer). -^ test was used to assess the relationship between EPAS~l/HIF-2ct and VEGF expression versus tumor grade and stage. Results The expressions of EPAS-l/HIF-2a and VEGF were negative in all normal bladder tissue but high in BTCC. Of the 60 cases,34 (56.6% ) were positive and 26 (43.4% ) were negative for EPASl/HIF-2a. Four cases (14.3%) of Grade 1,11 (91.7%) of Grade I and 19 (95.0%) of Grade II were positive for EPAS-l/HIF-2a. Positive staining was seen in 5 (17.2% ) of the superficial and 29 (93. 5% ) of the invasive cancer cases. EPAS-l/HIF-2ct positivity was correlated with high histological grade (r = 0. 862,P <0.001) and advanced pathological T stage (r = 0. 805 ,P <0. 001). Of the 60 oases,44 were positive and 16,negative for VEGF. Twelve cases (42. 8% ) of Grade I were positive; 12 (100% ) of Grade 2 and 20 (100% ) of Grade M were all positive for VEGF. Positive staining was seen in 14 (48.3% ) of the superficial and 30 (96. 8% ) of the invasive cancer cases. TEGF positivity was correlated with high histological grade (r =0. 819, P < 0.001). The expression of EPAS-1 /HIF-2a in invasive BTCC was closely correlated with VEGF (r = 0. 537.P < 0.001). Conclusions This study indicates that EPAS-l/HIF-2a expression is positively correlated with the occurrence and progression of BTCC. The exploration of its potential role can provide a diagnostic tool and possibly a target for gene therapy for invasive BTCC.
中文关键词	膀胱肿瘤 ; 癌 ; 低氧诱导因子2 ; 血管内皮生长因子
英文关键词	Bladder neoplasmas Carcinoma EPAS-l/HIF-2a VEGF
语种	中文
国家	中国
收录类别	CSCD
WOS类目	SURGERY
WOS研究方向	Surgery
CSCD记录号	CSCD:1682068
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/205834
作者单位	1.兰州医学院第二附属医院泌尿外科, 730030; 2.兰州医学院泌尿外科研究所
推荐引用方式 GB/T 7714	陈小勇,段建敏,卢建中,等. 低氧诱导因子2和血管内皮生长因子在膀胱癌中的表达及意义[J],2004,25(12):844-847.
APA	陈小勇,段建敏,卢建中,邱中瑾,&史庭凯.(2004).低氧诱导因子2和血管内皮生长因子在膀胱癌中的表达及意义.中华泌尿外科杂志,25(12),844-847.
MLA	陈小勇,et al."低氧诱导因子2和血管内皮生长因子在膀胱癌中的表达及意义".中华泌尿外科杂志 25.12(2004):844-847.