干旱区生态环境知识资源中心(Arid): 细胞非霍奇金淋巴瘤IgH基因重排检测及凝胶扫描技术应用的初步研究

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	细胞非霍奇金淋巴瘤IgH基因重排检测及凝胶扫描技术应用的初步研究
其他题名	Detection of IgH gene rearrangement in B-cell non-Hodgkin’s lymphoma and application of gel-scan method
	张晓飞 1; 周韧 2; 毛峥嵘 2; 骆利康 3; 胡孟钧 4; 杨水友 2; 李斐铭 5
来源期刊	中华病理学杂志
ISSN	0529-5807
出版年	2004
卷号	33 期号:6 页码:513-517
中文摘要	目的采用凝胶扫描技术,力求对采用聚合酶链反应(PCR)检测非霍奇金淋巴瘤(NHL)抗原受体基因重排结果分析时建立量化标准,以利于临床医师应用时客观判断,并为其筛选随访病例提供依据.方法用PCR 对96例B-NHL分别采用IgH FR3A及FR2A检测IgH基因克隆性重排.65例经IgH FR3APCR已证实为IgH基因克隆性重排B-NHL及8例良性病变淋巴组织和5例正常人外周血单核细胞,IgHFR3A PCR产物8%变性聚丙烯酰胺凝胶电泳银染后图像行凝胶扫描,绘制曲线并计算h1/h2比值.结果 (1)采用FR3A引物,克隆性IgH基因重排在96例B-NHL中检测率为68%,采用FR2A引物,检测率为61%,结合FR3A、FR2A引物,总检测率为83%.(2)凝胶扫描曲线示65例B-NHL的h1/h2均??3,而5例正常人外周血单核细胞曲线均表现为钟型,8例良性病变淋巴组织h1/h2比值均<1.5.结论非霍奇金淋巴瘤抗原受体基因重排检测的凝胶扫描分析曲线中,h1/h2峰高比值至少>3可提示为IgH基因克隆性重排,<1.5可能提示IgH基因多克隆重排.比值介于1.5和3之间,提示有随访意义.联合FR3A,FR2A引物,可提高B-NHL IgH基因克隆性重排的检测率.
英文摘要	Objective To develop a protocol for gene rearrangement study in non-Hodgkin’ s lymphoma (NHL) by PCR-directed gel-scan method and to set up quantitative criteria for IgH gene rearrangement which can be applied in the follow up of lymphoma patients. Methods IgH gene rearrangement studies were carried out in 96 cases of B-cell NHL. The detection rate of clonality was evaluated. Sixty-five cases of IgH gene rearranged cases proven by FR3A-directed PCR and PAGE and 8 cases of benign lymphoid tissues (5 cases of reactive lymphoid hyperplasia, 3 cases of chronic tonsillitis) , 5 cases of normal peripheral blood mononuclear cells were analyzed by gel-scan method and the proportion of hl/h2 (heights of peakl and peak2 of gel-scan) was calculated. Results The detection rate of IgH gene clonality was up to 68% using primer FR3A in the 96 B-cell NHL cases. The detection rate was up to 61% using primer FR2A. With a combination of primers FR3A and FR2A, the detection rate increased to 83% . Gel-scan curve showed that the value of hl/h2 was greater than 3 in all the 65 cases with IgH gene rearranged. In the 8 benign lymphoid tissue oases showed hl/h2 < 1. 5 , 5 cases with normal peripheral blood mononuclear cells showed a bell-shaped curve. Conclusions In the gel-soan curve of gene rearrangement studies in non-Hodgkin’s lymphoma samples, the value of hl/h2 greater than 3 represents a true clonal proliferation. The peaks with relative heights less than 1. 5 may not be significant and likely represent polyclonal cell population. A value between 1. 5 and 3 however requires clinical follow-up. The success rate of rearrangement studies in B-cell NHL can be increased by using a combination of primers FR3A and FR2A.
中文关键词	淋巴瘤,非霍奇金 ; 基因,免疫球蛋白 ; 基因重排,B淋巴细胞,重链
英文关键词	Lymphoma, non-Hodgkin Gene, immunoglobulin Gene rearrangement,B-lymphocyte, heavy chain
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:1695679
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/205812
作者单位	1.浙江大学医学院附属妇产科医院病理科; 2.浙江大学病理与法医学研究所, 杭州, 浙江 310006, 中国; 3.浙江省衢州市人民医院病理科; 4.诸暨市人民医院病理科; 5.东阳市人民医院
推荐引用方式 GB/T 7714	张晓飞,周韧,毛峥嵘,等. 细胞非霍奇金淋巴瘤IgH基因重排检测及凝胶扫描技术应用的初步研究[J],2004,33(6):513-517.
APA	张晓飞.,周韧.,毛峥嵘.,骆利康.,胡孟钧.,...&李斐铭.(2004).细胞非霍奇金淋巴瘤IgH基因重排检测及凝胶扫描技术应用的初步研究.中华病理学杂志,33(6),513-517.
MLA	张晓飞,et al."细胞非霍奇金淋巴瘤IgH基因重排检测及凝胶扫描技术应用的初步研究".中华病理学杂志 33.6(2004):513-517.