干旱区生态环境知识资源中心(Arid): 肉苁蓉总苷对D-半乳糖脑老化模型小鼠的保护作用

Arid

	肉苁蓉总苷对D-半乳糖脑老化模型小鼠的保护作用
其他题名	Protective effects of gloycosides of cistanche on the D-galactose induced brain aging model mice
	王新源 1; 王晓雯 2; 王雪飞 2; 蒋晓燕 2; 帕尔哈提 2
来源期刊	中国行为医学科学
ISSN	1005-8559
出版年	2004
卷号	13 期号:6 页码:613-615
中文摘要	目的探讨肉苁蓉总苷(GCs)对D-半乳糖(D-gal)脑老化模型小鼠的保护作用机制.方法实验分30d和50d,每期NIH小鼠各为120只,随机分为6组:正常对照组(NC)、模型组(D-gal)、VitaminE组、GCs组:分小、中、大剂量组[(mg.kg -1.d -1)31.25、62.5、125],每组20只.除NC组外,在各组小鼠皮下注射D-gal(50 mg.kg -1.d -1 );NC组皮下注射NS, D-gal组和NC组给予等容积饮水灌胃,VitaminE组和GCs组灌胃给药,每日1次,均连续皮下注射和灌胃30d和50d.分别测定5min错误次数、潜伏期、脑组织SOD的活性、MDA、LF含量,并观察50d后海马CA1区组织结构形态的变化.结果 (1)5min内的错误次数: 30d和50d的D-gal组分别为(8.254.03)次和(9.636.18)次,均显著高于其它各组(P 均＜ 0.01).(2)潜伏期: 30d和50d的D-gal组分别为 (25.2113.86)s和(31.75 17.77)s,低于NC组、GCs中剂量组和大剂量组(P ＜0.05,P ＜0.01).(3)SOD活性: 30d和50d D-gal组脑组织的SOD活性较其它各组低(P ＜0.05,P ＜0.01).(4)MDA含量:30d和50d D-gal组脑组织的MDA含量均较其它各组高(P ＜0.05,P ＜0.01).(5)LF含量:30d和50d D-gal组脑组织的LF含量均较NC组高,30d的脑组织GCs小剂量组、VitaminE组及50d GCs各组及VitaminE组均较D-gal组低(P ＜0.05,P ＜0.01).(6)50d的D-gal组小鼠海马CA1区与NC组、GCs大剂量组及VitaminE组的组织形态不同.结论 GCs能显著改善不同天数模型小鼠的学习、记忆功能,升高脑组织的SOD活性,降低MDA、LF含量,并对海马CA1区组织结构形态学改变有保护作用.提示GCs可能是通过抗氧化的机制达到改善脑老化的作用.
英文摘要	Objective To observe the influence of glycosides of cistanche (GCs) on the D-galactose in-duced brain aging model mice and explore the mechanism of GCs. Methods There were two stages including 30and 50 days. 120 NIH mice of every stage were randomly divided into six groups: normal control group (NC) ,model group (D-gal) , VitaminE group , GCs groups which was divided into lower dose, middle dose and largedose[ (mg. kg"1 d ’) 31.25, 62. 5 , 125 ]. Every group had 20 mice. Except for NC group, other groups were giv-en hypodermic injection of D-gal (50mg. kg’1. d"1). NC group was given hypodermic injection of NS. D-gal groupand NC group were given oral administration of water. VitaminE group and GCs groups were given oral administra-tion. Mice of various groups of every stage were repeatedly given hypodermic injection and oral administration onetime every day , and respectively measured erroneous number in 5 minutes , latent period and SOD activities,MDA content and LF content (brain ) after 30 and 50 days. Results (1) Compared with other groups, D-galgroups were obviously higher on erroneous number in 5 minutes which were 8. 25 4. 03 of 30 days and 9. 63 6. 18 of 50 days( P<0.01). (2) Compared with NC groups, middle dose and large dose groups of GCs, D-galgroups were lower on latent periods which were (25.21 13. 86)s of 30 days and (31.75 17.77)s of 50 days (P<0. 05 andP<0.01). (3) Compared with other groups, D-gal groups of brain tissue were lower on SOD activi-ties of 30 days and 50 days. ( P<0.05 andP<0.01). (4) Compared with other groups, D-gal groups of brainwere higher on MDA contents of 30 and 50 days. ( P<0.05 andP<0.01) . (5)Compared with NC groups, D-galgroups were higher on LF contents of brain tissue of 30 and 50 days. Compared with D-gal groups, lower dosegroup of GCs and VitaminE group of 30 days, and various groups o￡ GCs arid VitaminE group of 50 days were loweron LF contents of brain tissue ( P<0. 05 and P<0.01). (6)D-gal group was different from NC group .large dosegroup of GCs and VitaminiE group on hippocampus CAI region, of 50 days. Conclusions It indicated that GCscould improve the function of learning and memory’ , increase SOD activities and decrease MDA and LF contents ofbrain, the mechanism of which may relate with antioxidation.
中文关键词	肉苁蓉总苷 ; D-半乳糖 ; 脑老化模型 ; 学习和记忆 ; 海马CA1区 ; 抗氧化作用
英文关键词	Glycosides of cistanphe Dtgftlactose Brain aging model Learning and memory Hippocampus CAI region Antioxidation
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:1690030
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/205697
作者单位	1.新疆精神卫生中心精神科, 乌鲁木齐, 新疆 830002, 中国; 2.新疆医科大学药理教研室, 乌鲁木齐, 新疆 830002, 中国
推荐引用方式 GB/T 7714	王新源,王晓雯,王雪飞,等. 肉苁蓉总苷对D-半乳糖脑老化模型小鼠的保护作用[J],2004,13(6):613-615.
APA	王新源,王晓雯,王雪飞,蒋晓燕,&帕尔哈提.(2004).肉苁蓉总苷对D-半乳糖脑老化模型小鼠的保护作用.中国行为医学科学,13(6),613-615.
MLA	王新源,et al."肉苁蓉总苷对D-半乳糖脑老化模型小鼠的保护作用".中国行为医学科学 13.6(2004):613-615.