干旱区生态环境知识资源中心(Arid): 基因芯片用于检测beta地中海贫血

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	基因芯片用于检测beta地中海贫血
其他题名	Detection of beta-thalassemia by DNA microarray
	王沙燕 1; 蔡佩欣 2; 张阮章 1; 李芳芳 1; 张旭 3; 戴勇 1; 杨梦苏 2
来源期刊	中国地方病学杂志
ISSN	1000-4955
出版年	2004
卷号	23 期号:3 页码:262-265
中文摘要	目的探讨基因芯片诊断beta地中海贫血的方法.方法抽提样品基因组DNA,经聚合酶链反应后,进行荧光标记及杂交,扫描芯片荧光信号图像,将分析的结果与原基因类型进行对照.结果在40例被检样品中,正常个体4例,beta地贫杂合子29例,双重杂合子或纯合子7例.结论基因芯片法能同时快速、准确地筛查多种beta地中海贫血.
英文摘要	Objective To explore tlie techniques for genetic diagnosis of p-thalassemia using DNA microan-ay. Methods The assay was used to test a total of 40 blood archival samples. The genotypes had been determined previously. The genomic DNA was isolated from the blood. Sample DNA was amplified by the polymerase chain reaction (PCR), while a fluorescent label was incooperated and used as the probe for hybridization. Followed by scanned for the fluorescent signals showed on DNA microaiTay. The genotypes were scored before checking against the previously determined results. Results The diagnosis was definitely made in 40 samples, including 4 normal oases, 29 heterozygotes, 7 compound heterozygotes or homozygotea. The results were identical to that from previously determined genotypes. Conclusions DNA microaiTay technique is rapid and effective in screening the P-thalassemia.
中文关键词	beta-地中海贫血 ; 基因诊断 ; 基因芯片
英文关键词	p-thalassemia DNA microarray Gene diagnosis
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:1602914
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/205686
作者单位	1.暨南大学医学院附二院深圳市人民医院临床医学研究中心, 518020; 2.香港城市大学基因组应用中心; 3.深圳亚能生物技术有限公司
推荐引用方式 GB/T 7714	王沙燕,蔡佩欣,张阮章,等. 基因芯片用于检测beta地中海贫血[J],2004,23(3):262-265.
APA	王沙燕.,蔡佩欣.,张阮章.,李芳芳.,张旭.,...&杨梦苏.(2004).基因芯片用于检测beta地中海贫血.中国地方病学杂志,23(3),262-265.
MLA	王沙燕,et al."基因芯片用于检测beta地中海贫血".中国地方病学杂志 23.3(2004):262-265.