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	不同生境两种生态型芦苇叶片质膜H+-ATPase的比较
其他题名	Comparison of Plasma Membrane H+-ATPase Activity in Two Ecotypes of Reed (Phragmites communis) Leaves from Different Habitats
	敬岩; 宫海军; 赵志光; 陈国仓; 王锁民; 张承烈
来源期刊	植物学报
ISSN	1672-6650
出版年	2004
卷号	46 期号:9 页码:1040-1048
中文摘要	利用两相法纯化质膜微囊，研究了分布于西北沙漠地区的两种生态型芦苇(Phragmites communis Trin.)(水生芦苇和重度盐化草甸芦苇，分别简称为水芦和盐芦)叶片质膜H+-ATPase的部分性质。结果显示，与水芦相比，盐芦质膜H+-ATPase的ATP水解活性升高，Km值由1.27 mmol/L降至0.30 mmol/L，但Vmax没有显著差异。并且该酶活性对温度的敏感性和pH谱型也发生了变化。以对硝基苯磷酸盐为底物，低浓度时盐芦的质膜H+-ATPase水解活性高于水芦，高浓度时则没有差异。Km在水芦和盐芦中分别为3.61 mmol/L和1.92 mmol/L，但Vmax在两种生态型中没有差异。钒酸盐抑制实验表明，两种生态型的质膜H+-ATPase磷酸-酶区的催化性质不同。胰酶对质膜H+-ATPase活性的活化谱型也存在差异，说明该酶C末端的结构或性质发生了变化。此外，与水芦相比，盐芦质膜H+-ATPase的质子泵活性及与水解活性的耦联程度也升高了。以上结果说明，当芦苇从水生环境向盐渍环境过渡时，质膜H+-ATPase的催化性质发生了变化，这些变化可能是由酶结构的修饰和不同的同工酶谱引起的。H+-ATPase催化性质的变化可能是对盐渍生境的适应性反应.
英文摘要	Plasma membrane (PM) vesicles of the leaves of two ecotypes of reed (Phragmites communis Trin.), swamp reed (SR) and heavy salt meadow reed (HSMR) growing in the desert region of Northwest China, were purified by two-phase partitioning and the properties of their PM H+-ATPases (EC 3.6.1.35) were compared. The specific activity of this enzyme was greater in HSMR than in SR and the Km lower (1.27 mmol/L in SR and 0.30 mmol/L in HSMR), and the Vmax of ATP hydrolysis activity showed no significant difference between the two ecotypes. The PM H+-ATPase was more sensitive to denaturing temperatures in HSMR than in SR, and the pH profile also showed a slight difference, suggesting that the catalytic mechanism of this enzyme was different in HSMR compared with that in SR. The p -nitrophenyl phosphate (PNPP) hydrolysis activity of H+-ATPase was higher in HSMR than in SR at low concentrations of PNPP, but showed no difference at high PNPP concentration. The Km for PNPP hydrolysis was 3.61 mmol/L and 1.92 mmol/L in SR and HSMR, respectively. And the Vmax of PNPP hydrolysis showed no significant difference in the two reed ecotypes. An experiment with the inhibitor vanadate showed that the catalytic mechanisms of the phosphatase domain of the ATPase were different in the two ecotypes. The data obtained following trypsin treatment showed a difference in the enzyme activity pattern, suggesting that there existed a possible change in the C-terminus of the ATPase, either in the structure or in the property or both of them. In addition, compared with SR, the ATP-dependent H+ pumping activity of ATPase and the coupling between proton transport and ATP hydrolysis in HSMR were increased. These results indicated that the properties of PM H+-ATPase were changed in HSMR with compared to those in SR, which might include enzyme modifications and different isoforms expressed. The alterations of the properties of this enzyme might be an adaptive response to the habitat.
中文关键词	质膜 ; 芦苇生态型 ; 盐渍生境
英文关键词	plasma membrane (PM) H+-ATPase reed (Phragmites communis) ecotype saline habitat H+-ATPase
语种	英语
国家	中国
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:1843503
来源机构	兰州大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/205671
作者单位	Institute of Botany and Plant Physiology, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China
推荐引用方式 GB/T 7714	敬岩,宫海军,赵志光,等. 不同生境两种生态型芦苇叶片质膜H+-ATPase的比较[J]. 兰州大学,2004,46(9):1040-1048.
APA	敬岩,宫海军,赵志光,陈国仓,王锁民,&张承烈.(2004).不同生境两种生态型芦苇叶片质膜H+-ATPase的比较.植物学报,46(9),1040-1048.
MLA	敬岩,et al."不同生境两种生态型芦苇叶片质膜H+-ATPase的比较".植物学报 46.9(2004):1040-1048.