干旱区生态环境知识资源中心(Arid): 荔枝败育胚S-腺苷甲硫氨酸合成酶基因的全长扩增和序列分析

Arid

	荔枝败育胚S-腺苷甲硫氨酸合成酶基因的全长扩增和序列分析
其他题名	Full length Amplifying and Sequencing of S-adenosylmethionine Synthetase Gene in Litchi Aborted-embryo of ’Guiwei’
	张以顺 1; 向旭 2; 傅家瑞 1; 黄上志 1
来源期刊	园艺学报
ISSN	0513-353X
出版年	2004
卷号	31 期号:2 页码:160-164
中文摘要	从荔枝’桂味’的败育胚中提取总RNA，利用 GeneRace~(TM)Kit将RNA经走磷酸化反应、去 mRNA的帽子结构及RNA Oligo连接，反转录合成cDNA，以cDNA为模板，分别用SSH分离得到的胚败育相关差异表达S-腺苷甲硫氨酸合成酶基因的的部分cDNA序列(599 bp)设计出3’和5’端基因特异引物，进行PCR末端扩增。荔枝败育胚的S-腺苷甲硫氨酸合成酶基因序列全长为1515 bp，编码393个氨基酸，与其它物种的S-腺苷甲硫氨酸合成酶基冈的核苷酸序列同源性在79％～82％之间，氨基酸序列同源性在88％～91％之间。结果表明所获得的基因为与荔枝胚败育相关的S-腺苷甲硫氨酸合成酶基因。
英文摘要	Total RNA was extracted from aborted-embryo of litchi ’ Guiwei’. Dephosphorylating RNA, removing the mRNA cap structure and ligating the RNA Oligo to decapped mRNA with GeneRacer Kit and then the first strand of cDNA was synthesized by reverse transcription. The 3’. and 5’ gene specific primers from differential expressing cDNA fragments (SAM gene) , isolated from aborted-embryo of litchi varieties of Guiwei by SSH, was designed with Gene Tools. The full-length 3’ and 5’ ends of cDNA was amplified with the first cDNA as template and two gene specific primers or nested primers by PCR. Complete SAM gene sequence was obtained by BLAST comparison of the three fragments and splicing according to the overlapping regions. The sequence of the SAM synthetase gene is 1515 bp in length with an opening reading frame (ORF) encoding 393 amino acids. The nucleotide sequence exhibits about 79% -82% overall similarity to the corresponding gene of other organisms, arid the amino acids is about 88% -91%. It was suggested that the obtained cDNA was a SAM gene in aborted-embryo of litchi varieties of Guiwei.
中文关键词	荔枝
英文关键词	Litchi
语种	中文
国家	中国
收录类别	CSCD
WOS类目	HORTICULTURE
WOS研究方向	Agriculture
CSCD记录号	CSCD:1623994
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/205638
作者单位	1.中山大学生命科学学院, 广州, 广东 510275, 中国; 2.广东省农业科学院果树研究所, 广州, 广东 510640, 中国
推荐引用方式 GB/T 7714	张以顺,向旭,傅家瑞,等. 荔枝败育胚S-腺苷甲硫氨酸合成酶基因的全长扩增和序列分析[J],2004,31(2):160-164.
APA	张以顺,向旭,傅家瑞,&黄上志.(2004).荔枝败育胚S-腺苷甲硫氨酸合成酶基因的全长扩增和序列分析.园艺学报,31(2),160-164.
MLA	张以顺,et al."荔枝败育胚S-腺苷甲硫氨酸合成酶基因的全长扩增和序列分析".园艺学报 31.2(2004):160-164.