干旱区生态环境知识资源中心(Arid): AFLP分析小豆种（Vigna angularis）内遗传多样性

Arid

	AFLP分析小豆种（Vigna angularis）内遗传多样性
其他题名	Genetic Diversity in Vigna angularis Revealed by AFLP Analysis
	宗绪晓 1; 王新望 2; 关建平 1; 王述民 1
来源期刊	作物学报
ISSN	0496-3490
出版年	2003
卷号	29 期号:4 页码:562-568
中文摘要	利用12对ART引物，对来自于世界6个国家的146份小豆（Vigna angularis）栽培变种（var. angularis）和野生变种（var. nipponensis）种质的基因组DNA进行扩增，得到580条清晰的显带，其中313(53.93%）条呈多态性，平均每对AFLP引物得到26.08条多态性带；平均遗传距离0.35，变异幅度为0.00一0.87。利用AFLP多态性数据进行的Jaccard’，遗传距离聚类分析绘制的聚类图，可将其中的143份种质相互区分开，并将其中的145份小豆资源划分成8个明显不同组群，显示小豆种内存在足够的遗传多样性用于资源材料的准确鉴别与分类。8个组群的遗传多样性表现出十分明显的地域相关性，以及遗传类型趋同性。通过各组群内和组群间的遗传距离比较，发现世界小豆主要栽培资源以及日本野生、半野生资源中蓄积的遗传多样性较匮乏，而储藏于中国野生小豆资源、喜马拉雅地区栽培和野生资源中的遗传多样性较丰富。
英文摘要	A representalive set of 146 azuki ( Vigna angularis var. ungularis , and var. nipjionensin) germplasm from worldwide geographical regions, was used for AFLP analysis with 12 informative primer pairs. 580 unambiguous bands were created, 313 of which were polymorphic. One pair of AFLP primers has detected a mean of 26.08 polymorphic bands. All the 313 polymorphic bands were used to assess genetic distance among 146 azuki accessions to form the genetic distance matrix, by using Jaccard’ s method. The mean genetic distance among materials was 0.35 with a range o( 0.00 to 0.87 according to the matrix. Eight clusters were detected by using UPGMAM cluster analysis, based on the genetic distance matrix. According to the dentrograrn formed by cluster analysis, 143 from the whole materials were distinctly clarified, revealed enough genetic diversity for identification and classification of accessions within Vigna angularis . Eight clusters are clearly corresponded to the geographic origin of accessions. Comparing the genetic distances within and between clusters, the shortage of genetic diversity in the main cultivated accessions, as well as wild and weedy accessions from Japan is revealed ; Whilst the broader genetic diversity is shown in the wild accessions else where arid in the cultivated accessions from Himalayan area.
中文关键词	小豆（Vigna angularis） ; 种质资源 ; 遗传多样性
英文关键词	AFLP AFLP Vigna angularis Genetic resources Genetic diversity
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURAL ENGINEERING
WOS研究方向	Agriculture
CSCD记录号	CSCD:1321485
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/204765
作者单位	1.中国农业科学院作物品质资源研究所, 北京 100081, 中国; 2.日本农业生物资源研究所
推荐引用方式 GB/T 7714	宗绪晓,王新望,关建平,等. AFLP分析小豆种（Vigna angularis）内遗传多样性[J],2003,29(4):562-568.
APA	宗绪晓,王新望,关建平,&王述民.(2003).AFLP分析小豆种（Vigna angularis）内遗传多样性.作物学报,29(4),562-568.
MLA	宗绪晓,et al."AFLP分析小豆种（Vigna angularis）内遗传多样性".作物学报 29.4(2003):562-568.