干旱区生态环境知识资源中心(Arid): 肌细胞生成素在分化培养人体肌卫星细胞表达的研究

Arid

	肌细胞生成素在分化培养人体肌卫星细胞表达的研究
其他题名	Study of expression of myogenin Mrna during differentiation of human skeletal muscle satellite cells
	黄汉伟; 徐建光; 顾玉东; 胡韶楠; 李继峰; 沈燕国; 姜浩
来源期刊	中华手外科杂志
ISSN	1005-054X
出版年	2003
卷号	19 期号:3 页码:181-183
中文摘要	目的研究肌细胞生成素(myogenin)在人体肌卫星细胞分化培养中的表达规律,探索肌细胞生成素在肌卫星细胞分化中的作用.方法取6例正常成人的骨骼肌,消化、分离肌卫星细胞,肌卫星细胞生长培养后进行分化培养.在分化培养过程中,观察肌卫星细胞形态,计算其融合率,应用半定量RT-PCR技术检测细胞总RNA中肌细胞生成素 mRNA的表达量.结果在肌卫星细胞分化培养过程中,肌细胞生成素mRNA的表达增加,其规律与肌卫星细胞分化、融合相一致.结论肌细胞生成素参与人体肌卫星细胞分化过程.
英文摘要	Objective To investigate the expression rule and its function of myogenin mRNA during differentiation of human skeletal muscle satellite cells. Methods Six oases of healthy human skeletal muscles were gained from patients during orthopedics operation. Muscle satellite cells were cultivated in differentiation medium for 5 days after they were cultured in growth medium. During differentiation, the morphology of muscle satellite cell was observed and the fusion rate was calculated under microscopy. The level of myogenin mRNA expression was checked by semi-quantitative RT-PCR. Results During skeletal muscle satellite differentiation and culture, the expression of myogenin mRNA was elevated in accordance with the differentiation and fusion. Conclusion Myogenin plays a role on the differentiation of human skeleted muscle satellite cells.
中文关键词	细胞,培养的 ; 肌形成蛋白 ; 肌卫星细胞 ; 聚合酶链式反应
英文关键词	Cells cultured Myogenin Skeletal muscle satellite cells Polymerase chain reaction
语种	中文
国家	中国
收录类别	CSCD
WOS类目	SURGERY
WOS研究方向	Surgery
CSCD记录号	CSCD:1487225
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/204731
作者单位	复旦大学华山医院手外科, 上海 200040, 中国
推荐引用方式 GB/T 7714	黄汉伟,徐建光,顾玉东,等. 肌细胞生成素在分化培养人体肌卫星细胞表达的研究[J],2003,19(3):181-183.
APA	黄汉伟.,徐建光.,顾玉东.,胡韶楠.,李继峰.,...&姜浩.(2003).肌细胞生成素在分化培养人体肌卫星细胞表达的研究.中华手外科杂志,19(3),181-183.
MLA	黄汉伟,et al."肌细胞生成素在分化培养人体肌卫星细胞表达的研究".中华手外科杂志 19.3(2003):181-183.